Peripheral orphanin FQ/Nociceptin analgesia in the mouse Journal Article

  


Authors: Kolesnikov, Y. A.; Pasternak, G. W.
Article Title: Peripheral orphanin FQ/Nociceptin analgesia in the mouse
Abstract: Orphanin FQ/Nociceptin (OFQ/N) administered peripherally was an effective analgesic in the tailflick test in mice (ED50 16.3 μg). It had a peak effect at 5 min and lasted up to 30 min. The kappa3 analgesic naloxone benzoylhydrazone was also active peripherally (ED50 3.8 μg). The analgesic actions of both agents were blocked by naloxone. Neither OFQ/N(1-11) nor OFQ/N(1-7) had appreciable peripheral activity. Antisense mapping both compounds against the murine orphan opioid receptor (KOR-3) confirmed the importance of this clone in their actions. Antisense probes targeting the second and third coding exons significantly lowered the analgesic effects of both compounds. However, the antisense targeting the first coding exon blocked only the actions of OFQ/N and not kappa3 analgesia.
Keywords: controlled study; exons; nonhuman; mouse; animals; mice; animal experiment; nerve tissue proteins; neuromodulation; mice, inbred icr; animalia; murinae; base sequence; analgesics, opioid; pain measurement; opioid; injections, subcutaneous; analgesia; receptor binding; nociception; tail flick test; naloxone; opioid receptor; opioid peptides; naloxone benzoylhydrazone; kappa opiate receptor; nociceptin; narcotic antagonists; receptors, opioid; topical; male; article; nociceptin receptor; antisense elements (genetics); orl1; anti-opioid; kor-3
Journal Title: Life Sciences
Volume: 64
Issue: 22
ISSN: 0024-3205
Publisher: Elsevier Inc.  
Date Published: 1999-04-23
Start Page: 2021
End Page: 2028
Language: English
DOI: 10.1016/s0024-3205(99)00149-6
PUBMED: 10374927
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033597386&partnerID=40&md5=38875c0b8ae54c26269386ac22080a97
Notes: Article -- Export Date: 16 August 2016 -- Source: Scopus
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MSK Authors
  1. Yuri Alexei Kolesnikov
    34 Kolesnikov
  2. Gavril W Pasternak
    414 Pasternak
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