A role for ADP ribosylation factor in the control of cargo uptake during COPI-coated vesicle biogenesis Journal Article
| Authors: | Malsam, J.; Gommel, D.; Wieland, F. T.; Nickel, W. |
| Article Title: | A role for ADP ribosylation factor in the control of cargo uptake during COPI-coated vesicle biogenesis |
| Abstract: | ARF-mediated hydrolysis of GTP has been demonstrated to regulate coat disassembly of Golgi-derived COPI transport vesicles (Tanigawa, G., Orci, L., Amherdt, M., Ravazzola, M., Helms, J.B. and Rothman, J.E. (1993) J. Cell Biol. 123, 1365-1371). In addition, a requirement for GTP hydrolysis at an early stage of COPI vesicle biogenesis has been established since cargo uptake is impaired in the presence of GTPĪ³S (Nickel, W., Malsam, J., Gorgas, K., Ravazzola, M., Jenne, N., Helms, J.B. and Wieland, F.T. (1998) J. Cell Sci. 111, 3081-3090), a non-hydrolyzable analogue of GTP. We now demonstrate that the GTPase involved in the regulation of cargo uptake is ARF, revealing a multi-functional role of this GTPase in COPI-mediated vesicular transport. The molecular mechanism of cargo uptake as well as the functional implications of these findings on the overall process of COPI vesicle biogenesis are discussed. Copyright (C) 1999 Federation of European Biochemical Societies. |
| Keywords: | mutation; nonhuman; animals; cell line; cell transport; hydrolysis; biogenesis; golgi complex; cho cells; cricetinae; adenosine diphosphate ribosylation; guanosine triphosphatase; intracellular membranes; vesicular transport; guanosine 5'-o-(3-thiotriphosphate); coat protein complex i; coated vesicle; rabbit; cargo; coated vesicles; golgi apparatus; coat protein; golgi; adp-ribosylation factors; coatomer; priority journal; article; adp ribosylation factor; copi-coated vesicle |
| Journal Title: | FEBS Letters |
| Volume: | 462 |
| Issue: | 3 |
| ISSN: | 0014-5793 |
| Publisher: | Wiley Blackwell |
| Date Published: | 1999-12-03 |
| Start Page: | 267 |
| End Page: | 272 |
| Language: | English |
| DOI: | 10.1016/s0014-5793(99)01543-4 |
| PUBMED: | 10622709 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
