CD8+ T cell cytolytic activity independent of mitogen-activated protein kinase/extracellular regulatory kinase signaling (MAP kinase/ERK) Journal Article
| Authors: | Lilić, M.; Kulig, K.; Messaoudi, I.; Remus, K.; Janković, M.; Nikolić-Žugić, J.; Vukmanović, S. |
| Article Title: | CD8+ T cell cytolytic activity independent of mitogen-activated protein kinase/extracellular regulatory kinase signaling (MAP kinase/ERK) |
| Abstract: | Cytotoxicity is a major effector function of CD8+ T cells. Although mitogen-activated protein kinase (MAP kinase)/extracellular regulatory kinase (ERK) activity is indispensable for cytotoxic activity of most CD8+ T cells a portion of CD8 T cells appears resistant to MEK inhibition as cytotoxicity of bulk cultures was partially preserved in the presence of a MEK inhibitor. We have also identified a long-term CD8+ T cell line with unaltered cytolytic activity after prevention of ERK activation. Antigen-induced microtubule organizing center (MTOC) reorientation was not prevented in this CD8+ cell line by MEK inhibition, in sharp contrast to the MTOC reorientation prevention in a CD8+ T cell clone with MEK inhibition-sensitive cytolytic activity. These findings suggest that resistance of lysis to MEK inhibition may be due to a lack of ERK control over MTOC reorientation in some CD8+ T cells. Thus, there appears to be a heterogeneity of ERK-regulated cytolytic activity in CD8+ T cells, most likely resulting from a differential control of ERK over MTOC motility. |
| Keywords: | signal transduction; mitogen activated protein kinase; controlled study; nonhuman; cd8 antigen; t lymphocyte; cd8-positive t-lymphocytes; animal cell; animals; mice; map kinase signaling system; cytotoxicity; mice, inbred balb c; mice, inbred c57bl; ctl; cytolysis; cytotoxicity, immunologic; mitogen-activated protein kinases; microtubule; antigens, cd8; map kinase; erk; priority journal; article |
| Journal Title: | European Journal of Immunology |
| Volume: | 29 |
| Issue: | 12 |
| ISSN: | 0014-2980 |
| Publisher: | Wiley V C H Verlag Gmbh |
| Date Published: | 1999-12-01 |
| Start Page: | 3971 |
| End Page: | 3977 |
| Language: | English |
| DOI: | 10.1002/(sici)1521-4141(199912)29:12<3971::aid-immu3971>3.0.co;2-5 |
| PUBMED: | 10602006 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
