GM-CSF rescues TF-1 cells from growth factor withdrawal-induced, but not differentiation-induced apoptosis: The role of BCL-2 and MCL-1 Journal Article
| Authors: | Klampfer, L.; Zhang, J.; Nimer, S. D. |
| Article Title: | GM-CSF rescues TF-1 cells from growth factor withdrawal-induced, but not differentiation-induced apoptosis: The role of BCL-2 and MCL-1 |
| Abstract: | Cytokines such as granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-3 promote the survival and stimulate the proliferation of haematopoietic cells. Using the GM-CSF-dependent TF-1 myeloid leukaemia cell line, the authors show that the endogenous levels of BCL-2 and MCL-1 are downregulated upon GM-CSF withdrawal, whereas the levels of BCLx(L) and Bax are unchanged. Re-exposure of growth factor deprived cells to GM-CSF resulted in an early and transient increase in MCL-1 expression, and prolonged induction of BCL-2, which prevented apoptosis. In contrast, the expression of BCL-2 and MCL-1 were not modulated during TPA-induced differentiation of TF-1 cells, which was followed by apoptosis despite the presence of GM-CSF. TF-1 cells overexpressing BCL-2 or MCL-1 underwent delayed apoptosis upon growth factor withdrawal, but displayed no impaired apoptosis in response to TPA. Erythropoietin (Epo) induced the expression of BCL-2 and MCL-1 protein in TF-1 cells, however it did not support their long term proliferation, further demonstrating that upregulation of these anti-apoptotic genes is insufficient for the long term proliferation of TF-1 cells. |
| Keywords: | controlled study; protein expression; human cell; cell proliferation; cell survival; protein bcl 2; apoptosis; erythropoietin; granulocyte macrophage colony stimulating factor; neoplasm proteins; cell differentiation; tumor cells, cultured; transfection; time factors; gene expression regulation; leukemia, myeloid; protein induction; proto-oncogene proteins c-bcl-2; tetradecanoylphorbol acetate; gm-csf; bcl-2; growth substances; protein bax; granulocyte-macrophage colony-stimulating factor; interleukin 3; protein bcl x; mcl-1; humans; human; priority journal; article |
| Journal Title: | Cytokine |
| Volume: | 11 |
| Issue: | 11 |
| ISSN: | 1043-4666 |
| Publisher: | Academic Press, Elsevier Inc |
| Date Published: | 1999-11-01 |
| Start Page: | 849 |
| End Page: | 855 |
| Language: | English |
| DOI: | 10.1006/cyto.1999.0514 |
| PUBMED: | 10547272 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
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