| Abstract: |
We studied the effect of hematopoietic growth factors (granulocyte- macrophage colony-stimulating factor [GM-CSF], granulocyte [G]-CSF, interleukin (IL)-1, IL-3, IL-5, IL-6, and macrophage [M]-CSF) on differentiation and functional activity of human eosinophilic HL-60 cells (Eos-HL-60) and compared them with effects on parental HL-60 promyelocytic leukemia cells. Purified biosynthetic GM-CSF and IL-5 enhanced cell proliferation and induced eosinophilic differentiation in the eosinophilic subline in both liquid and agar cultures. IL-3 and IL-6 stimulated cell proliferation but had no effect on cell differentiation, whereas IL-1 and G- CSF affected neither differentiation nor proliferation of Eos-HL-60 cells under the conditions tested. GM-CSF-, IL-3-, and IL-5-treated Eos-HL-60 cells showed increased O2- production in response to phorbol esters (PMA), enhanced phagocytosis of Candida albicans, and release of the enzymes arylsulfatase, β-glucuronidase and eosinophil peroxidase (EPO). The degranulation of eosinophils induced by GM-CSF, IL-5, and IL-3 may have relevance to the potential clinical toxicity of these hematopoietins, which also stimulate eosinophilopoiesis. G-CSF had no effect on enzyme release, oxidative metabolism, or phagocytic capacity of Eos-HL-60 cells. IL-5 did not affect proliferation, differentiation, or enzyme release in promyelocytic HL- 60 cells. These results indicate the specificity of IL-5 for the eosinophil lineage, confirm the effects of GM-CSF and IL-3 on eosinophilopoiesis and mature eosinophil function in a model system, and indicate the absence of G- CSF and IL-1 stimulation of eosinophils. The Eos-HL-60 line is a useful model for studying human eosinophil responses to cytokines. |
| Keywords: |
controlled study; human cell; cell function; cell growth; cell line; cell differentiation; tumor cells, cultured; leukemia, promyelocytic, acute; kinetics; recombinant proteins; promyelocytic leukemia; interleukin 6; interleukin-6; phagocytosis; superoxides; enzyme release; granulocyte colony stimulating factor; granulocyte colony-stimulating factor; recombinant granulocyte macrophage colony stimulating factor; candida albicans; colony stimulating factor 1; interleukin-1; recombinant interleukin 5; granulocyte-macrophage colony-stimulating factor; eosinophil; eosinophils; aerobic metabolism; interleukin 3; interleukin-3; granulocytes; cell strain hl 60; peroxidases; macrophage colony-stimulating factor; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; support, u.s. gov't, non-p.h.s.; hematopoietic cell growth factors; interleukin-5; recombinant interleukin 1alpha
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