Functional interaction between retinoblastoma protein and stress-activated protein kinase in multiple myeloma cells Journal Article
| Authors: | Chauhan, D.; Hideshima, T.; Treon, S.; Teoh, G.; Raje, N.; Yoshihimito, S.; Tai, Y. T.; Li, W.; Fan, J.; DeCaprio, J.; Anderson, K. C. |
| Article Title: | Functional interaction between retinoblastoma protein and stress-activated protein kinase in multiple myeloma cells |
| Abstract: | Previous studies have demonstrated that y-irradiation (IR)-induced apoptosis in multiple myeloma (MM) is associated with activation of stress- activated protein kinase (SAPK). In the present study, we examined the molecules downstream of SAPK/C-Jun N-terminal kinase (JNK), focusing on the role of retinoblastoma protein (Rb) during IR-induced MM cell apoptosis. The results demonstrate that IR activates SAPK/JNK, which associates with Rb both in vivo and in vitro. Far Western blot analysis confirms that SAPK/JNK binds directly to Rb. IR-activated SAPK/JNK phosphorylates Rb, and deletion of the phosphorylation site in the COOH terminus domain of Rb abrogates phosphorylation of Rb by SAPK/JNK. Taken together, our results suggest that Rb is a target protein of SAPK/JNK and that the association of SAPK/JNK and Rb mediates IR-induced apoptosis in MM cells. |
| Keywords: | signal transduction; human cell; apoptosis; multiple myeloma; stress activated protein kinase; protein protein interaction; protein binding; enzyme activation; tumor cells, cultured; phosphorylation; gamma irradiation; mitogen-activated protein kinases; retinoblastoma protein; jnk mitogen-activated protein kinases; gamma rays; ca(2+)-calmodulin dependent protein kinase; humans; human; priority journal; article |
| Journal Title: | Cancer Research |
| Volume: | 59 |
| Issue: | 6 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1999-03-15 |
| Start Page: | 1192 |
| End Page: | 1195 |
| Language: | English |
| PUBMED: | 10096546 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
