Cyclooxygenase-2 expression is up-regulated in squamous cell carcinoma of the head and neck Journal Article
| Authors: | Chan, G.; Boyle, J. O.; Yang, E. K.; Zhang, F.; Sacks, P. G.; Shah, J. P.; Edelstein, D.; Soslow, R. A.; Koki, A. T.; Woerner, B. M.; Masferrer, J. L.; Dannenberg, A. J. |
| Article Title: | Cyclooxygenase-2 expression is up-regulated in squamous cell carcinoma of the head and neck |
| Abstract: | The purpose of this study was to determine whether cyclooxygenase-2 (COX-2) was overexpressed in squamous cell carcinoma of the head and neck (HNSCC). Quantitative reverse transcription-PCR, immunoblotting, and immunohistochemistry were used to assess the expression of COX-2 in head and neck tissue. Mean levels of COX-2 mRNA were increased by nearly 150-fold in HNSCC (n = 24) compared with normal oral mucosa from healthy volunteers (n = 17). Additionally, there was about a 50-fold increase in amounts of COX-2 mRNA in normal-appearing epithelium adjacent to HNSCC (n = 10) compared with normal oral mucosa from healthy volunteers. Immunoblotting demonstrated that COX-2 protein was present in six of six cases of HNSCC but was undetectable in normal oral mucosa from healthy subjects. Immunohistochemical analysis showed that COX-2 was expressed in both HNSCC and adjacent normal-appearing epithelium. Taken together, these results suggest that COX-2 may be a target for the prevention or treatment of HNSCC. |
| Keywords: | immunohistochemistry; human tissue; protein expression; squamous cell carcinoma; carcinoma, squamous cell; cell proliferation; reverse transcription polymerase chain reaction; membrane proteins; transcription, genetic; immunoreactivity; gene expression regulation, neoplastic; reverse transcriptase polymerase chain reaction; head and neck neoplasms; rna, messenger; cyclooxygenase 2; reference values; epithelium cell; target cell; dna primers; mouth mucosa; head and neck carcinoma; isoenzymes; gene expression regulation, enzymologic; receptor upregulation; prostaglandin-endoperoxide synthases; early response gene; humans; human; priority journal; article |
| Journal Title: | Cancer Research |
| Volume: | 59 |
| Issue: | 5 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1999-03-01 |
| Start Page: | 991 |
| End Page: | 994 |
| Language: | English |
| PUBMED: | 10070952 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
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