Adoptive-transfer therapy of tumors with the tumor-specific primary cytotoxic T cells induced in vitro with the B7.1-transduced MCA205 cell line Journal Article
| Authors: | Kim, S. J.; Sadelain, M.; Lee, J. S.; Seong, R. H.; Yun, Y. S.; Jang, Y. J.; Chung, H. Y. |
| Article Title: | Adoptive-transfer therapy of tumors with the tumor-specific primary cytotoxic T cells induced in vitro with the B7.1-transduced MCA205 cell line |
| Abstract: | We show that the tumor-specific primary cytotoxic T lymphocytes (CTL) induced in vitro with the MCA205 fibrosarcoma cells transduced with the B7.1 (CD80) gene are highly effective in adoptive-transfer therapy of the parental tumors. The MCA205 fibrosarcoma cell line was transduced with the retroviral vectors encoding the B7.1 gene and tested for their efficiency as stimulators in short-term (5 days) mixed lymphocyte/tumor cell cultures with highly purified syngenic, unprimed T cells as responders. The induction of the CTL required the presence of a low dose of interleukin-2 (25 U/ml). The injection of the CTL prevented colony formation by the intravenously injected tumor cells in a lung colonization assay in which the CTL were injected after inoculation of tumor cells. We also showed that the adoptive transfer of the same T cells was effective in delaying the growth of the subcutaneously injected tumor cells. These results imply that the short-term mixed lymphocyte/tumor cell culture with the tumor cells transduced with the gene for the B7.1 costimulatory molecule is potentially a good source of CTL for adoptive-transfer therapy of tumors. |
| Keywords: | controlled study; nonhuman; animal cell; mouse; animals; mice; interleukin 2; cancer immunotherapy; animal experiment; animal model; antineoplastic activity; mice, inbred balb c; mice, inbred c57bl; lung tumor; mixed lymphocyte culture; fibrosarcoma; immunotherapy; tumors; cytotoxic t lymphocyte; t-lymphocytes, cytotoxic; limb tumor; adoptive transfer; immunotherapy, adoptive; b7 antigen; retrovirus; virus vector; antigens, cd80; sarcoma, experimental; priority journal; article; b7.1; primary cytotoxic t lymphocytes |
| Journal Title: | Cancer Immunology, Immunotherapy |
| Volume: | 47 |
| Issue: | 5 |
| ISSN: | 0340-7004 |
| Publisher: | Springer |
| Date Published: | 1999-01-01 |
| Start Page: | 257 |
| End Page: | 264 |
| Language: | English |
| DOI: | 10.1007/s002620050529 |
| PUBMED: | 10022469 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
