Induction of human cytotoxic T lymphocytes by artificial antigen- presenting cells Journal Article

Authors: Latouche, J. B.; Sadelain, M.
Article Title: Induction of human cytotoxic T lymphocytes by artificial antigen- presenting cells
Abstract: The adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) is a promising therapeutic approach for a number of diseases. To overcome the difficulty in generating specific CTLs, we established stable artificial antigen-presenting cells (AAPCs) that can be used to stimulate T cells of any patient of a given human leukocyte antigen (HLA) type. Mouse fibroblasts were retrovirally transduced with a single HLA-peptide complex along with the human accessory molecules B7.1, ICAM-1, and LFA-3. These AAPCs consistently elicit strong stimulation and expansion of HLA-restricted CTLs. Owing to the high efficiency of retrovirus-mediated gene transfer, stable AAPCs can be readily engineered for any HLA molecule and any specific peptide.
Keywords: human cell; nonhuman; cd8-positive t-lymphocytes; t-lymphocytes; animal cell; mouse; animals; mice; transfection; animalia; genetic vectors; lymphocyte activation; dendritic cells; immunotherapy; recombinant proteins; cytotoxic t lymphocyte; t-lymphocytes, cytotoxic; fibroblast; adoptive cell therapy; adoptive transfer; intercellular adhesion molecule 1; lymphocyte function associated antigen 3; antigen presenting cell; hla antigen; antigen-presenting cells; t lymphocyte activation; b7 antigen; retroviridae; 3t3 cells; intercellular adhesion molecule-1; hla-a2 antigen; costimulation; cd8+ t cell; unidentified retrovirus; humans; human; priority journal; article; hla-b7 antigen; retrovirus-mediated gene transfer; antigens, cd58
Journal Title: Nature Biotechnology
Volume: 18
Issue: 4
ISSN: 1087-0156
Publisher: Nature Publishing Group  
Date Published: 2000-04-01
Start Page: 405
End Page: 409
Language: English
DOI: 10.1038/74455
PUBMED: 10748520
PROVIDER: scopus
Notes: Export Date: 18 November 2015 -- Source: Scopus
Citation Impact
MSK Authors
  1. Michel W J Sadelain
    543 Sadelain