Induction of human cytotoxic T lymphocytes by artificial antigen- presenting cells Journal Article
| Authors: | Latouche, J. B.; Sadelain, M. |
| Article Title: | Induction of human cytotoxic T lymphocytes by artificial antigen- presenting cells |
| Abstract: | The adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) is a promising therapeutic approach for a number of diseases. To overcome the difficulty in generating specific CTLs, we established stable artificial antigen-presenting cells (AAPCs) that can be used to stimulate T cells of any patient of a given human leukocyte antigen (HLA) type. Mouse fibroblasts were retrovirally transduced with a single HLA-peptide complex along with the human accessory molecules B7.1, ICAM-1, and LFA-3. These AAPCs consistently elicit strong stimulation and expansion of HLA-restricted CTLs. Owing to the high efficiency of retrovirus-mediated gene transfer, stable AAPCs can be readily engineered for any HLA molecule and any specific peptide. |
| Keywords: | human cell; nonhuman; cd8-positive t-lymphocytes; t-lymphocytes; animal cell; mouse; animals; mice; transfection; animalia; genetic vectors; lymphocyte activation; dendritic cells; immunotherapy; recombinant proteins; cytotoxic t lymphocyte; t-lymphocytes, cytotoxic; fibroblast; adoptive cell therapy; adoptive transfer; intercellular adhesion molecule 1; lymphocyte function associated antigen 3; antigen presenting cell; hla antigen; antigen-presenting cells; t lymphocyte activation; b7 antigen; retroviridae; 3t3 cells; intercellular adhesion molecule-1; hla-a2 antigen; costimulation; cd8+ t cell; unidentified retrovirus; humans; human; priority journal; article; hla-b7 antigen; retrovirus-mediated gene transfer; antigens, cd58 |
| Journal Title: | Nature Biotechnology |
| Volume: | 18 |
| Issue: | 4 |
| ISSN: | 1087-0156 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2000-04-01 |
| Start Page: | 405 |
| End Page: | 409 |
| Language: | English |
| DOI: | 10.1038/74455 |
| PUBMED: | 10748520 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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