α-Tocopherol dietary supplement decreases titers of antibody against 5- hydroxymethyl-2'-deoxyuridine (HMdU) Journal Article
| Authors: | Hu, J. J.; Chi, C. X.; Frenkel, K.; Smith, B. N.; Henfelt, J. J.; Berwick, M.; Mahabir, S.; D'Agostino, R. B. Jr |
| Article Title: | α-Tocopherol dietary supplement decreases titers of antibody against 5- hydroxymethyl-2'-deoxyuridine (HMdU) |
| Abstract: | This study evaluated the effects of vitamin E (α-tocopherol) on oxidative DNA damage in a randomized double-blind Phase II chemoprevention trial. Oxidative DNA damage was measured by the level of auto-antibody (Ab) against 5-hydroxymethyl-2'-deoxyuridine (HMdU) in plasma. After the baseline screening, eligible subjects (n = 31; plasma samples from 28 subjects were available for this study) were randomized to receive 15, 60, or 200 mg of α- tocopherol per day for 28 days. Biomarkers were measured twice at baseline - on day 1 (visit 1) and day 3 (visit 2) - and twice after intervention - on day 17 (visit 3) and day 31 (visit 4). At baseline, there was a highly significant inverse correlation between anti-HMdU Ab titer and plasma vitamin E level (r = -0.53; P = 0.004; n = 28). Smoking did not affect baseline anti- HMdU Ab titer; however, anti-HMdU Ab titer levels at baseline were significantly lower in subjects with above-median (0.75 ounce/day) alcohol consumption (P = 0.008). No significant change in anti-HMdU Ab level occurred at either visit 3 or visit 4 for subjects on the lowest dose, 15 mg α- tocopherol per day. Subjects receiving 60 mg of α-tocopherol per day had a significant decrease in anti-HMdU Ab level at visits 3 and 4 compared with baseline (P = 0.049 and P = 0.02, respectively). However, subjects receiving the highest dose, 200 mg/day, had less consistent results: a significant decrease in anti-HMdU Ab level was seen at visit 4 (P = 0.04) but not at visit 3. Our results demonstrate an inverse relationship between α- tocopherol and anti-HMdU Abs in plasma; oxidative DNA damage can be modulated by short-term dietary supplementation of α-tocopherol in some subjects. |
| Keywords: | adult; controlled study; middle aged; human cell; clinical trial; neoplasms; cell proliferation; dna damage; dna repair; controlled clinical trial; randomized controlled trial; dose-response relationship, drug; diet supplementation; reactive oxygen species; dietary supplements; alpha tocopherol; alcohol consumption; double-blind method; frozen section; lipid peroxidation; autoantibodies; environmental exposure; antibody titer; metabolic regulation; lipid solubility; vitamin e; biochemical marker; humans; human; male; female; priority journal; article; 5 hydroxymethyldeoxyuridine; deoxyuridine |
| Journal Title: | Cancer Epidemiology Biomarkers and Prevention |
| Volume: | 8 |
| Issue: | 8 |
| ISSN: | 1055-9965 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1999-08-01 |
| Start Page: | 693 |
| End Page: | 698 |
| Language: | English |
| PUBMED: | 10744129 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
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