Isoforms of the human PDZ-73 protein exhibit differential tissue expression Journal Article
| Authors: | Scanlan, M. J.; Williamson, B.; Jungbluth, A.; Stockert, E.; Arden, K. C.; Viars, C. S.; Gure, A. O.; Gordan, J. D.; Chen, Y. T.; Old, L. J. |
| Article Title: | Isoforms of the human PDZ-73 protein exhibit differential tissue expression |
| Abstract: | Patients with renal and colon cancer frequently develop IgG autoantibodies toward the NY-CO-38/PDZ-73 antigen, a protein of 652 amino acids (73 kDa) which contains three copies of the PDZ protein-protein interaction domain. The gene encoding PDZ-73 mapped to chromosome 11p15.4- p15.1. Additional tissue-specific isoforms were identified: PDZ-45, which lacks the third PDZ domain and the putative PEST protein degradation motif, is expressed in kidney, colon, small intestine, brain and testis; PDZ-54 and PDZ-59, which also lack the third PDZ domains, have unique carboxyl terminal amino acids and are expressed in brain, kidney, bladder, colon cancer and renal cancer; and a putative PDZ-37 isoform, containing only the third PDZ domain, that is expressed in the central nervous system. Immunohistochemical staining with anti-PDZ 73 monoclonal antibodies showed strong cytoplasmic reactivity in epithelial cells of the small intestine, colon and kidney tubules, with a prominent apical staining pattern in cells of the small intestine. The reactivity pattern of the antibodies with various tissues correlated with the mRNA expression pattern of the PDZ-45 isoform. The existence of multiple PDZ-73 isoforms with variations in tissue distribution, PDZ domains, protein degradation sequences and carboxyl terminal structure indicate that these isoforms have distinct tissue-specific functions. |
| Keywords: | immunohistochemistry; protein expression; unclassified drug; human cell; protein domain; carboxy terminal sequence; protein degradation; protein protein interaction; colonic neoplasms; kidney neoplasms; cloning, molecular; dna; amino acid sequence; molecular sequence data; antigen; brain; rna, messenger; colon cancer; nucleotide sequence; carrier proteins; adaptor proteins, signal transducing; base sequence; dna sequence; autoantibody; intestine, small; testis; protein isoforms; immunoglobulin g antibody; chromosome mapping; dna, complementary; tissue specificity; chromosome 11p; pdz domain; humans; human; male; priority journal; article; 11p15.4-p15.1; isoform expression; ny-co-38 antigen; serological expression cloning; protein pdz 73 |
| Journal Title: | Biochimica Et Biophysica Acta (BBA) - Gene Structure and Expression |
| Volume: | 1445 |
| Issue: | 1 |
| ISSN: | 0167-4781 |
| Publisher: | Elsevier B.V. |
| Date Published: | 1999-04-14 |
| Start Page: | 39 |
| End Page: | 52 |
| Language: | English |
| DOI: | 10.1016/s0167-4781(99)00033-0 |
| PUBMED: | 10209257 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
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