Helix pomatia lectin, an inducer of Drosophila immune response, binds to hemomucin, a novel surface mucin Journal Article
| Authors: | Theopold, U.; Samakovlis, C.; Erdjument-Bromage, H.; Dillon, N.; Axelsson, B.; Schmidt, O.; Tempst, P.; Hultmark, D. |
| Article Title: | Helix pomatia lectin, an inducer of Drosophila immune response, binds to hemomucin, a novel surface mucin |
| Abstract: | We describe the isolation and initial characterization of hemomucin, a novel Drosophila surface mucin that is likely to be involved in the induction of antibacterial effector molecules after binding a snail lectin (Helix pomatia A hemagglutinin). Two proteins of 100 and 220 kDa were purified from the membrane fraction of a Drosophila blood cell line using lectin columns. The two proleins are products of the same gene, as demonstrated by peptide sequencing. The corresponding cDNAs code for a product that contains an amino-terminal putative transmembrane domain, a domain related to the plant enzyme strictosidine synthase, and a mucin-like domain in the carboxyl- terminal part of the protein. The gene is expressed throughout development. In adult flies, high expression is found in hemocytes, in specialized regions of the gut, and in the ovary, where the protein is deposited onto the egg surface. In the gut, the mucin co-localizes with the peritrophic membrane. The cytogenetic location of the gene is on the third chromosome in the region 97F-98A. |
| Keywords: | sequence analysis; nonhuman; protein analysis; animal cell; animals; carboxy terminal sequence; cell line; protein binding; membrane proteins; drosophila; cytogenetics; animalia; gene expression regulation; cloning, molecular; immune response; amino acid sequence; molecular sequence data; sequence homology, amino acid; amino terminal sequence; cell membrane; peptides; base sequence; blood cell; genome; drosophila proteins; subcellular fractions; chromosomal localization; protein isolation; dna, complementary; mucin; mucins; antibacterial activity; synthetase; lectin; lectins; antimicrobial cationic peptides; gastropoda; priority journal; article; strictosidine; helix pomatia |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 271 |
| Issue: | 22 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1996-05-31 |
| Start Page: | 12708 |
| End Page: | 12715 |
| Language: | English |
| DOI: | 10.1074/jbc.271.22.12708 |
| PUBMED: | 8662683 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
