Up-regulation of MDC15 (metargidin) messenger RNA in human osteoarthritic cartilage Journal Article
| Authors: | Böhm, B. B.; Aigner, T.; Gehrsitz, A.; Blobel, C. P.; Kalden, J. R.; Burkhardt, H. |
| Article Title: | Up-regulation of MDC15 (metargidin) messenger RNA in human osteoarthritic cartilage |
| Abstract: | Objective. The aim of the study was to investigate the messenger RNA (mRNA) expression of the disintegrin metalloproteinase MDC15 (metargidin, or ADAM-15) in normal and osteoarthritic (OA) articular cartilage. Methods. In situ hybridization experiments and reverse transcription-polymerase chain reaction (RT-PCR) were performed on tissue samples of adult normal and OA articular cartilage. Results. MDC15 mRNA could be detected in normal articular cartilage by RT-PCR using tissue-extracted total RNA as a template. However, the mRNA level remained below the sensitivity of in situ hybridization. In contrast, in situ hybridizations of OA cartilage revealed an intense staining with the MDC15-specific riboprobes. The extension of the analysis to chondrosarcomas showed a strong up-regulation of MDC15 mRNA in these malignant transformed cells. Conclusion. Our results demonstrate a markedly strong up-regulation of MDC15 in adult OA and neoplastic cartilage compared with adult normal articular cartilage, indicating a potential role of the disintegrin metalloproteinase in cartilage remodeling. |
| Keywords: | adult; clinical article; controlled study; aged; middle aged; human cell; reverse transcription polymerase chain reaction; membrane proteins; in situ hybridization; messenger rna; reverse transcriptase polymerase chain reaction; rna, messenger; protein induction; osteoarthritis; up-regulation; chondrosarcoma; enzyme synthesis; adam proteins; sequence analysis, dna; disintegrin; articular cartilage; disintegrins; metalloendopeptidases; humans; human; priority journal; article; cartilage, articular |
| Journal Title: | Arthritis and Rheumatism |
| Volume: | 42 |
| Issue: | 9 |
| ISSN: | 0004-3591 |
| Publisher: | Wiley Blackwell |
| Date Published: | 1999-09-01 |
| Start Page: | 1946 |
| End Page: | 1950 |
| Language: | English |
| DOI: | 10.1002/1529-0131(199909)42:9<1946::aid-anr21>3.0.co;2-e |
| PUBMED: | 10513811 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
