| Abstract: |
GD3 Synthase (α2,8sialyltransferase) (EC 2.4.99.8) cDNA has been cloned by eukaryotic cell expression cloning. Using this cDNA as a probe, the expression level of the gene in human cancer cell lines was analysed by Northern blotting and RT-PCR, then correlated with the ganglioside expression and enzyme activity. Melanoma cell lines showed extremely strong bands in Northern blot and RT-PCR/Southern analysis. The enzyme activity was also very high in melanomas as expected. Neuroblastoma and astrocytoma lines showed relatively low levels of the gene expression, whereas they expressed high levels of GD2. Although the mRNA level of the GD3 synthase gene and enzyme activity in individual cell lines correlated positively, some cell lines showed much higher activity than expected from the mRNA level. Among leukaemia lines, adult T cell leukaemia-associated (HTLV-I+) lines showed fairly high levels of the mRNA. On the other hand, T-ALL lines showed very low levels. In addition, GD3 and GD2 expression and mRNA level of the gene during T lymphocyte activation were analysed. Only GD3 expression was induced by any of the stimulatory reagents used, and corresponding up-regulation of the GD3 synthase gene was shown in RT-PCR/Southern analysis. © 1995 Chapman & Hall. |
| Keywords: |
controlled study; human cell; polymerase chain reaction; t-lymphocytes; melanoma; gene expression; cell line; transcription, genetic; tumor cells, cultured; gene expression regulation, neoplastic; lymphocyte activation; molecular sequence data; ganglioside gd2; messenger rna; neuroblastoma cell; eukaryota; leukemia cell; tumor cell; sialyltransferase; sialyltransferases; melanoma cell; base sequence; immunophenotyping; immunoassay; up-regulation; blotting, northern; t lymphocyte activation; ganglioside gd3; t lymphocytes; gene expression regulation, enzymologic; gangliosides; melanomas; astrocytoma cell; humans; human; priority journal; article; α2,8-sialyltransferase gene; gd3 synthase gene
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