Separation of extra- and intracellular metabolites using hyperpolarized (13)C diffusion weighted MR Journal Article
| Authors: | Koelsch, B. L.; Sriram, R.; Keshari, K. R.; Swisher, C. L.; Van Criekinge, M.; Sukumar, S.; Vigneron, D. B.; Wang, Z. J.; Larson, P. E. Z.; Kurhanewicz, J. |
| Article Title: | Separation of extra- and intracellular metabolites using hyperpolarized (13)C diffusion weighted MR |
| Abstract: | This work demonstrates the separation of extra- and intracellular components of glycolytic metabolites with diffusion weighted hyperpolarized 13C magnetic resonance spectroscopy. Using b-values of up to 15,000 s mm−2, a multi-exponential signal response was measured for hyperpolarized [1-13C] pyruvate and lactate. By fitting the fast and slow asymptotes of these curves, their extra- and intracellular weighted diffusion coefficients were determined in cells perfused in a MR compatible bioreactor. In addition to measuring intracellular weighted diffusion, extra- and intracellular weighted hyperpolarized 13C metabolites pools are assessed in real-time, including their modulation with inhibition of monocarboxylate transporters. These studies demonstrate the ability to simultaneously assess membrane transport in addition to enzymatic activity with the use of diffusion weighted hyperpolarized 13C MR. This technique could be an indispensible tool to evaluate the impact of microenvironment on the presence, aggressiveness and metastatic potential of a variety of cancers. © 2016 |
| Keywords: | diffusion; enzyme activity; renal cell carcinoma; magnetic resonance; magnetic resonance spectroscopy; biomolecules; diseases; resonance; magnetism; pyruvate; metabolites; lactate; curve fitting; aerobic glycolysis; hyperpolarized; cancer; diffusion weighted magnetic resonance; cancer aggressiveness; dynamic nuclear polarization (dnp); hyperpolarized 13c magnetic resonance (hp 13c mr); lactate efflux; renal cell carcinoma (rcc); dynamic nuclear polarization; pyruvates; cellular transport; diffusion-weighted magnetic resonance |
| Journal Title: | Journal of Magnetic Resonance |
| Volume: | 270 |
| ISSN: | 1090-7807 |
| Publisher: | Academic Press Inc., Elsevier Science |
| Date Published: | 2016-09-01 |
| Start Page: | 115 |
| End Page: | 123 |
| Language: | English |
| DOI: | 10.1016/j.jmr.2016.07.002 |
| PROVIDER: | scopus |
| PUBMED: | 27434780 |
| PMCID: | PMC5448422 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 2 August 2016 -- Source: Scopus |
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