Generation of TCR-like antibodies using phage display Journal Article
| Authors: | Santich, B. H.; Liu, H.; Liu, C.; Cheung, N. K. V. |
| Article Title: | Generation of TCR-like antibodies using phage display |
| Abstract: | The adaptive immune response against cancer consists of two arms: the humoral response from B cells, and the cell-mediated response from T cells. The humoral response has the advantage of diversity, theoretically recognizing antigens of any type (sugar, protein, lipid, etc.), but is generally limited to surface -expressed targets. T cells on the other hand, can recognize intracellular targets, but only if they are proteins, and presented as small peptide fragments on major histocompatibility complex (MHC) cell surface antigens. However, with advances in protein engineering and phage display, it has become feasible to quickly identify and generate antibodies or single -chain variable fragments against peptide-MHC, thus bridging the two arms, and allowing for recognition, identification, and effector responses against cells expressing intracellular targets. |
| Keywords: | protein expression; dna; antigens; ligands; antibody; phage display; molecules; selection; complex; zinc fingers; human leukocyte antigen; scfv; major histocompatibility; single-chain; phage; t-cell receptor; fc-fusion protein; variable fragment; peptide mhc; fab library |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 1348 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Date Published: | 2015-01-01 |
| Start Page: | 191 |
| End Page: | 204 |
| Language: | English |
| ACCESSION: | WOS:000369818200018 |
| PROVIDER: | wos |
| PUBMED: | 26424273 |
| DOI: | 10.1007/978-1-4939-2999-3_17 |
| Notes: | Chapter 17 in "Peptide Antibodies: Methods and Protocols" (ISBN: 978-1-4939-2998-6) -- Source: Wos |
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