Identification of a naturally processed NY-ESO-1 peptide recognized byCD8+T cells in the context of HLA-B51 Journal Article

Authors: Jäger, E.; Karbach, J.; Gnjatic, S.; Jäger, D.; Maeurer, M.; Atmaca, A.; Arand, M.; Skipper, J.; Stockert, E.; Chen, Y. T.; Old, L. J.; Knuth, A.
Article Title: Identification of a naturally processed NY-ESO-1 peptide recognized byCD8+T cells in the context of HLA-B51
Abstract: NY-ESO-1 is one of the most immunogenic cancer antigens known to date, inducing humoral and cellular immune responses in a high proportion of patients with advanced NY-ESO-1-expressing cancers. The assessment of spontaneous and vaccine-induced CD8+ T cell responses has been limited to a small number of known NY-ESO-1 epitopes presented by MHC class I alleles. Recently, a new method to monitor NY-ESO-1-specific CD8+ T cell responses was introduced that does not depend on the individual MHC class I status and on predefined peptide epitopes. Antigen-presenting cells transduced with recombinant adenoviral vectors encoding NY-ESO-1 were used to stimulate CD8+ selected NY-ESO-1-specific T cells. Effector cells were tested for recognition of autologous B cell targets transfected with NY-ESO-1 using a recombinant vaccinia virus construct. Using a modified approach we identified the NY-ESO-1 p94-102 peptide as being recognized by CD8+ T cells in the context of HLA- B51. NY-ESO-1 p94-102 specific CD8+ T cells recognized naturally processed NY-ESO-1 presented by HLA-B51+ monocyte-derived dendritic and tumor cells. Transfection of target cells with NY-ESO-1 combined with different HLA class I alleles confirmed that the NY-ESO-1 peptide was naturally processed and recognized by HLA-B51-restricted CD8+ T cell lines and clones. Therefore, NY-ESO-1 p94-102 is a new candidate peptide antigen for cancer immunotherapy and for the monitoring of spontaneous and vaccine-induced NY-ESO-1-specific T cell responses in HLA- B51+ patients with NY-ESO-1 expressing malignancies.
Keywords: unclassified drug; human cell; t lymphocyte; dendritic cell; cytotoxicity; b lymphocyte; genetic transduction; genetic transfection; cancer vaccine; molecular recognition; antigen recognition; tumor cell; peptides; adenovirus vector; ex vivo study; t lymphocyte activation; major histocompatibility antigen class 1; ny-eso-1; peptide analysis; human; article; cytotoxic t lymphocytes; hla-b51; hla b51 antigen; ny eso 1 antigen[94-102]
Journal Title: Cancer Immunity
ISSN: 1424-9634
Publisher: Academy of Cancer Immunology  
Date Published: 2002-09-19
Language: English
PROVIDER: scopus
PUBMED: 12747757
Notes: Export Date: 14 November 2014 -- Source: Scopus