Radioligand binding assay for an exon 11-associated mu opioid receptor target Journal Article
| Authors: | Marrone, G. F.; Majumdar, S.; Pasternak, G. W. |
| Article Title: | Radioligand binding assay for an exon 11-associated mu opioid receptor target |
| Abstract: | Receptor binding provides a valuable approach for characterization of drugs and their receptors. There are three major families of opioid receptors: mu, delta, and kappa. Highly selective radioligands are available for all three classes of traditional receptors. Of the three, the mu receptor undergoes extensive alternative splicing, generating a number of traditional mu receptor subtypes as well as a nontraditional, truncated set of variants associated with exon 11. These exon 11-associated truncated variants are not readily labeled with current radioligands. Here we describe the synthesis of a radioiodinated ligand suitable for carrying out binding studies for this target. © Springer Science+Business Media New York 2015. |
| Keywords: | exon; nonhuman; drug targeting; mouse; animal tissue; drug synthesis; drug receptor binding; alternative rna splicing; morphine; opioid; ligand binding; mu opiate receptor; mor-1; radioligand; gpcr; mu receptor; priority journal; article; ibntxa; receptor binding assay |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 1335 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Date Published: | 2015-01-01 |
| Start Page: | 241 |
| End Page: | 249 |
| Language: | English |
| DOI: | 10.1007/978-1-4939-2914-6_16 |
| PROVIDER: | scopus |
| PUBMED: | 26260605 |
| DOI/URL: | |
| Notes: | Chapter 16 in "G Protein-Coupled Receptors in Drug Discovery" (ISBN: 978-1-4939-2913-9) -- Export Date: 21 June 2016 -- Source: Scopus |
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