Isolation and expression of a novel alternatively spliced mu opioid receptor isoform, MOR-1F Journal Article
| Authors: | Pan, Y. X.; Xu, J.; Bolan, E.; Chang, A.; Mahurter, L.; Rossi, G.; Pasternak, G. W. |
| Article Title: | Isolation and expression of a novel alternatively spliced mu opioid receptor isoform, MOR-1F |
| Abstract: | The MOR-1 gene is large, with a recent study reporting nine exons spanning 250 kb which combine to yield six different mu opioid receptor splice variants. We now report the isolation of exon 10, which is contained within yet another splice variant, MOR-1F, which is composed of exons 1, 2, 3, 10, 6, 7, 8 and 9. Exon 10 comprises 186 bp which predict a unique 58 amino acid sequence extending beyond exon 3. It has been mapped between exons 4 and 6 and has flanking consensus splice sequences. On Northern blot analysis, the MOR-1F mRNA is smaller than the other MOR-1 mRNAs. When expressed in CHO cells, MOR-1F binds the mu opioid radioligand [3H]DAMGO with high affinity (K(D)=1.04±0.03 nM). Competition studies demonstrated the selectivity of the variant for mu opioid ligands, supporting its classification within the mu opioid receptor family. (C) 2000 Federation of European Biochemical Societies. |
| Keywords: | sequence analysis; exons; nonhuman; mouse; animals; mice; gene expression; molecular cloning; gene mapping; amino acid sequence; molecular sequence data; reverse transcriptase polymerase chain reaction; nucleotide sequence; alternative splicing; protein biosynthesis; base sequence; dna flanking region; morphine; analgesia; mu opiate receptor; receptors, opioid, mu; protein isoforms; antisense mapping; rna splicing; dna, complementary; codon, terminator; splicing; cho cells; cricetinae; mor-1; gene isolation; male; priority journal; article; morphine-6β-glucuronide |
| Journal Title: | FEBS Letters |
| Volume: | 466 |
| Issue: | 2-3 |
| ISSN: | 0014-5793 |
| Publisher: | Wiley Blackwell |
| Date Published: | 2000-01-28 |
| Start Page: | 337 |
| End Page: | 340 |
| Language: | English |
| DOI: | 10.1016/s0014-5793(00)01095-4 |
| PUBMED: | 10682855 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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