Cloning and expression of a new splice variant, mMOR-1R, of the mouse mu-opioid receptor gene Meeting Abstract


Authors: Pan, Y. X.; Xu, L.; Mahurter, L.; Xu, M. M.; Pasternak, G. W.
Abstract Title: Cloning and expression of a new splice variant, mMOR-1R, of the mouse mu-opioid receptor gene
Meeting Title: 32nd Annual Meeting of the Society for Neuroscience
Abstract: Our previous studies has identified and characterized fourteen splice variants of the mouse mu-opioid receptor (MOR-1) gene. Here we report isolation of an additional mouse MOR-1 splice variant, mMOR-1R, using an RT-PCR strategy. Sequence analysis indicates that mMOR-1R contains another new exon, exon 15, with an exon composition of 1, 2, 3 and 15. Exon 15 has been mapped to downstream of the exon 3 with similar location as the fourth exon of hMOR-1R. Exon 15 is predicted to encode 70 amino acids in the C-terminal of the mMOR-1R, which contains putative phosphorylation sites for both cAMP-and cGMP-dependent protein kinase and protein kinase C. Northern blot analysis of mouse brain total RNA using an exon 15 probe showed a distinct band, approximately 1.8 kb. Expression of the mMOR-1R in mouse brain was also determined by Western blot analysis with a specific mMOR-1R antibody. Pharmacological studies in CHO cells stably transfected with the mMOR-1R cDNA suggested that mMOR-1R encodes a mu-opioid receptor.
Journal Title: Society for Neuroscience Abstract Viewer and Itinerary Planner
Volume: 22
Meeting Dates: 2002 Nov 3-7
Meeting Location: Orlando, FL
ISSN: 1558-3635
Publisher: Society for Neuroscience  
Date Published: 2002-01-01
Language: English
ACCESSION: BCI:BCI200300314809
PROVIDER: biosis
Notes: Meeting Abstract: 554.1/E-98 -- 32nd Annual Meeting of the Society for Neuroscience -- Orlando, Florida, USA -- Society for Neuroscience -- Source: Biosis