Simultaneous quantitation of lamotrigine, levetiracetam, 10-hydroxycarbazepine, topiramate, and zonisamide in serum using HPLC-MS/MS Journal Article
| Authors: | Carlow, D. C.; Shi, H.; Schofield, R. C. |
| Article Title: | Simultaneous quantitation of lamotrigine, levetiracetam, 10-hydroxycarbazepine, topiramate, and zonisamide in serum using HPLC-MS/MS |
| Title Series: | Methods in Molecular Biology |
| Abstract: | Antiepileptic drugs (AEDs) are a diverse group of pharmacological agents used in the treatment of epileptic seizures. Over the past several decades some new AEDs, including lamotrigine (LTG), levetiracetam (LVA), oxcarbazepine (OXC), topiramate (TOP), and zonisamide (ZNS), have become widely used. This chapter describes a very simple and rapid liquid chromatography-tandem mass spectrometry method for simultaneous quantitation of LVA, ZNS, LTG, TOP, and MHD in human serum. The method requires a very small amount of serum (50 mu L) for multiple drug measurements and has a total analysis time of 4 min that makes it well suited for routine clinical analysis of several drugs simultaneously. The imprecision (CVs) measured at various concentrations across the analytical measurement range (AMR) are less than 7 % for all analytes. The AMR for each analyte is as follows: LVA (1-100 mu g/mL), ZNS (0.8-80 mu g/mL), TOP (0.5-50 mu g/mL), and 0.6-60 mu g/mL for LTG and MHD. |
| Keywords: | mass spectrometry; topiramate; antiepileptic drugs; lamotrigine; zonisamide; levetiracetam; therapeutic drug monitoring; 10-hydroxycarbazepine; newer antiepileptic drugs |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 1383 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Publication Place: | New York, NY |
| Date Published: | 2016-01-01 |
| Start Page: | 29 |
| End Page: | 37 |
| Language: | English |
| ACCESSION: | WOS:000368837100005 |
| PROVIDER: | wos |
| PUBMED: | 26660171 |
| DOI: | 10.1007/978-1-4939-3252-8_4 |
| Notes: | Article; Book Chapter 4 in "Clinical Applications of Mass Spectrometry in Drug Analysis: Methods and Protocols" (ISBN: 978-1-4939-3251-1) -- 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA -- Source: Wos |
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