Isolated single-lung perfusion with TNF-α in a rat sarcoma lung metastases model Conference Paper
| Authors: | Weksler, B.; Blumberg, D.; Lenert, J. T.; Ng, B.; Fong, Y. M.; Burt, M. E. |
| Title: | Isolated single-lung perfusion with TNF-α in a rat sarcoma lung metastases model |
| Conference Title: | 30th Annual Meeting of the Society of Thoracic Surgeons |
| Abstract: | We conducted a trial of isolated lung perfusion using tumor necrosis factor (TNF) in an experimental sarcoma lung metastasis model. In an in vitro experiment, methylcholanthrene-induced sarcoma cells were incubated for 48 hours with 42 mu g/mL of either human or murine TNF. Controls were incubated with Hank's balanced salt solution. In an in vivo experiment, 23 F344 rats were injected with 10(7) methylcholanthrene-induced sarcoma cells. On day 7, 4 animals were perfused with 210 mu g of murine TNF, 5 animals were perfused with 420 mu g of murine TNF, 10 animals underwent isolated lung perfusion with 420 mu g of human TNF; and 4 animals were injected systemically with 420 mu g of human TNF. Animals were sacrificed on day 14 and the lung nodules counted. The cells incubated with murine TNF exhibited a 21% decrease in growth (p = 0.07); cells incubated with human TNF showed a 37% decrease in growth (p < 0.05). Animals perfused with 210 mu g/mL of murine TNF and animals treated by systemically administered human TNF showed no tumor response. Animals perfused with 420 mu g/mL of murine TNF had 7.8 +/- 14.2 nodules on the left lung and 58.5 +/- 66.0 nodules on the right lung (p = 0.07). Animals perfused with 420 mu g/mL of human TNF had 21.7 +/- 18.3 nodules on the left lung and 91.7 +/- 66.2 nodules on the right lung (p < 0.01). On the basis of these findings, we conclude that isolated lung perfusion with TNF can be done safely in the rat and is effective in decreasing the growth of sarcoma lune metastases. |
| Keywords: | shock; injury; tumor-necrosis-factor; combination; cachectin; gamma-interferon; cancer |
| Journal Title | Annals of Thoracic Surgery |
| Volume: | 58 |
| Issue: | 2 |
| Conference Dates: | 1994 Jan 31-Feb 2 |
| Conference Location: | New Orleans, LA |
| ISBN: | 0003-4975 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 1994-08-01 |
| Start Page: | 328 |
| End Page: | 331; discussion 332 |
| Language: | English |
| ACCESSION: | WOS:A1994PD58900010 |
| DOI: | 10.1016/0003-4975(94)92202-0 |
| PROVIDER: | wos |
| PUBMED: | 8067827 |
| Notes: | Article; Proceedings Paper -- Source: Wos |
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