Abundant primary piRNAs, endo-siRNAs, and microRNAs in a Drosophila ovary cell line Journal Article
| Authors: | Lau, N. C.; Robine, N.; Martin, R.; Chung, W. J.; Niki, Y.; Berezikov, E.; Lai, E. C. |
| Article Title: | Abundant primary piRNAs, endo-siRNAs, and microRNAs in a Drosophila ovary cell line |
| Abstract: | Piwi proteins, a subclass of Argonaute-family proteins, carry ∼24-30-nt Piwi-interacting RNAs (piRNAs) that mediate gonadal defense against transposable elements (TEs). We analyzed the Drosophila ovary somatic sheet (OSS) cell line and found that it expresses miRNAs, endogenous small interfering RNAs (endo-siRNAs), and piRNAs in abundance. In contrast to intact gonads, which contain mixtures of germline and somatic cell types that express different Piwi-class proteins, OSS cells are a homogenous somatic cell population that expresses only PIWI and primary piRNAs. Detailed examination of its TE-derived piRNAs and endo-siRNAs revealed aspects of TE defense that do not rely upon ping-pong amplification. In particular, we provide evidence that a subset of piRNA master clusters, including flamenco, are specifically expressed in OSS and ovarian follicle cells. These data indicate that the restriction of certain TEs in somatic gonadal cells is largely mediated by a primary piRNA pathway. © 2009 by Cold Spring Harbor Laboratory Press. |
| Keywords: | controlled study; gene cluster; nonhuman; animal cell; animals; microrna; gene amplification; gene expression; models, biological; cell line; small interfering rna; rna, small interfering; gene locus; drosophila; cell population; ovary follicle; gene expression regulation; ovary; molecular sequence data; nucleotide sequence; base sequence; micrornas; sequence homology, nucleic acid; gene location; piwi interacting rna; gonad; ovary cell; transposon; rna precursors |
| Journal Title: | Genome Research |
| Volume: | 19 |
| Issue: | 10 |
| ISSN: | 1088-9051 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Date Published: | 2009-10-01 |
| Start Page: | 1776 |
| End Page: | 1785 |
| Language: | English |
| DOI: | 10.1101/gr.094896.109 |
| PUBMED: | 19541914 |
| PROVIDER: | scopus |
| PMCID: | PMC2765285 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 16" - "Export Date: 30 November 2010" - "CODEN: GEREF" - "Source: Scopus" |
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