Detection of microscopic disease: Comparing histology, immunocytology, and RT-PCR of tyrosine hydroxylase, GAGE, and MAGE Journal Article
| Authors: | Cheung, I. Y.; Cheung, N. K. V. |
| Article Title: | Detection of microscopic disease: Comparing histology, immunocytology, and RT-PCR of tyrosine hydroxylase, GAGE, and MAGE |
| Abstract: | Background. We first explored the use of multiple molecular markers to overcome tumor heterogeneity. Sixty-seven neuroblastoma (NB) tumors were tested for the expression of GAGE, MAGE-1, MAGE-2, MAGE-3, and MAGE-4 by RT-PCR and then chemiluminescence; 82% of tumors had detectable GAGE, and 88% expressed at least one of the four MAGE genes. Procedure and Results. By combining GAGE and MAGE, 64 of 67 (95%) of tumors became detectable; 17 of 67 coexpressed all five molecular markers. Neither GAGE nor MAGE expression correlated with stage. GAGE was found to have the broadest (18 of 18) expression among stage 4 tumors. Two hundred fifty-nine bone marrows from 99 patients were then studied for NB positivity by four detection methods: Histology, immunocytology, and molecular detection by GAGE and tyrosine hydroxylase (TH) mRNA. Two hundred seven samples were NB-positive by one detection method. All four techniques were comparable in detecting tumor cells at diagnosis and at relapse. GAGE and immunocytology were far more sensitive than histology and TH mRNA when marrows were sampled during chemotherapy and at the time of clinical remission. Conclusions. By combining multiple molecular markers and independent screening techniques, we may be able to overcome tumor heterogeneity and expedite the detection of microscopic disease in the clinical management of neuroblastoma. © 2001 Wiley-Liss, Inc. |
| Keywords: | immunohistochemistry; human tissue; disease-free survival; major clinical study; histopathology; conference paper; cancer diagnosis; neoplasm staging; sensitivity and specificity; reverse transcription polymerase chain reaction; gene expression; neoplasm proteins; tumor markers, biological; algorithms; histology; cancer genetics; gene expression regulation, neoplastic; antibodies, monoclonal; antigens, neoplasm; immunocytology; neuroblastoma; tyrosine 3 monooxygenase; neoplasm, residual; reverse transcriptase polymerase chain reaction; rna, messenger; remission induction; intermethod comparison; rna, neoplasm; bone marrow examination; cytodiagnosis; dna, complementary; biotinylation; rt-pcr; gangliosides; mage; chemiluminescent measurements; tyrosine 3-monooxygenase; chemoluminescence; gage; humans; human; priority journal; microscopic disease; tyrosine hydroxylase |
| Journal Title: | Medical and Pediatric Oncology |
| Volume: | 36 |
| Issue: | 1 |
| ISSN: | 0098-1532 |
| Publisher: | Wiley Liss |
| Date Published: | 2001-01-01 |
| Start Page: | 210 |
| End Page: | 212 |
| Language: | English |
| DOI: | 10.1002/1096-911x(20010101)36:1<210::aid-mpo1051>3.0.co;2-f |
| PUBMED: | 11464887 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Conference Paper -- Export Date: 22 February 2016 -- Source: Scopus |
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