Gene profiling approach in the analysis of lymphotoxin and TNF deficiencies Journal Article
| Authors: | Shakhov, A. N.; Lyakhov, I. G.; Tumanov, A. V.; Rubtsov, A. V.; Drutskaya, L. N.; Marino, M. W.; Nedospasov, S. A. |
| Article Title: | Gene profiling approach in the analysis of lymphotoxin and TNF deficiencies |
| Abstract: | Mice with combined lymphotoxin-alpha (LT alpha) and tumor necrosis factor (TNF) deficiencies show defects hi the structure of peripheral lymphoid organs such as spleen, lymph nodes, and gut-associated lymphoid tissues. To identify genes associated with this defective phenotype in spleen. we applied a gene profiling approach, including subtractive cloning and gene array hybridizations, to mice with combined TNF/LT deficiency. The differentially expressed genes identified by these techniques was then evaluated by Northern blot analysis for splenic expression ill knockout mice with single LT alpha or single TNF deficiency. Most of the genes detected in this analysis are directly or indirectly associated with disrupted LT and not TNF signaling. |
| Keywords: | mice; spleen; b-cell; expression; tumor-necrosis-factor; tissues; responses; cloning; immune; alpha; knockout mice; gene arrays; subtractive cloning; lymphoid organ development |
| Journal Title: | Journal of Leukocyte Biology |
| Volume: | 68 |
| Issue: | 1 |
| ISSN: | 0741-5400 |
| Publisher: | Federation of American Societies for Experimental Biology |
| Date Published: | 2000-07-01 |
| Start Page: | 151 |
| End Page: | 157 |
| Language: | English |
| ACCESSION: | WOS:000088080600021 |
| PROVIDER: | wos |
| PUBMED: | 10914503 |
| Notes: | Article; Proceedings Paper -- 8th Conference of the International-Cytokine-Society -- DEC 05-10, 1999 -- HILTON HEAD ISL, SOUTH CAROLINA -- Source: Wos |
