Limiting dilution analysis of TNF producing cells in C3H/HeJ mice Journal Article
| Authors: | Lattime, E. C.; Stoppacciaro, A.; Stutman, O. |
| Article Title: | Limiting dilution analysis of TNF producing cells in C3H/HeJ mice |
| Abstract: | A limiting dilution assay (LDA) that measures the frequency of TNF producing cells is described. LDA determination is based on the inhibition of growth of a highly TNF sensitive subline from the WEHI-164 fibrosarcoma by using a micro assay sensitive to single picogram amounts of recombinant murine TNF. Using such LDA, it was determined that the reported deficiency in LPS-induced TNF production in C3H/HeJ mice is a function of reduced frequency of TNF producing cells rather than a complete lack of responsiveness. In bulk culture, LPS-triggered TNF was produced by Thy-1.2 negative spleen cells with activity recovered in both G10 Sephadex adherent and nonadherent subpopulations. LPS stimulation of spleen cells from C3H/HeJ mice resulted in TNF mRNA expression as shown in both Northern blots and in situ hybridization. The frequency of TNF mRNA bearing cells in control of C3H/HeSnJ mice by in situ hybridization correlated with that found for TNF producing cells in LDA. In C3H/HeJ spleen, significantly higher numbers of TNF mRNA positive cells were found than were shown to produce TNF in LDA. |
| Keywords: | nonhuman; animal cell; mouse; phenotype; animal; mice; spleen; cell line; animal experiment; mice, inbred balb c; b lymphocyte; fibrosarcoma; cell culture; rna, messenger; leukocyte count; antigens, surface; tumor necrosis factor; mice, inbred c3h; frequency analysis; cytotoxicity tests, immunologic; lipopolysaccharides; antigens, thy-1; female; priority journal; support, u.s. gov't, p.h.s. |
| Journal Title: | Journal of Immunology |
| Volume: | 141 |
| Issue: | 10 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 1988-11-15 |
| Start Page: | 3422 |
| End Page: | 3428 |
| Language: | English |
| PUBMED: | 2903192 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 6 August 2020 -- Source: Scopus |
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