Screening for genetic aberrations in papillary thyroid cancer by using comparative genomic hybridization Journal Article

Authors: Singh, B.; Lim, D. ; Cigudosa, J. C.; Ghossein, R.; Shaha, A. R.; Poluri, A.; Wreesmann, V. B.; Tuttle, M.; Shah, J. P.; Rao, P. H.
Article Title: Screening for genetic aberrations in papillary thyroid cancer by using comparative genomic hybridization
Abstract: Background. Determination of the genetic composition of papillary thyroid cancers may help explain differences in observed clinical behavior. Comparative genomic hybridization (CGH) is a novel molecular cytogenetic assay that allows simultaneous detection of gains, losses, and amplification of genetic information, making it an ideal screening tool. The aim of this study was to identify genetic aberrations occurring in papillary thyroid cancers by using CGH analysis. Methods. CGH analysis was performed on 21 individual cases of papillary thyroid cancers. Nonparametric statistical comparisons were performed with the Fisher exact test. Results. Genetic abnormalities were identified by CGH in 10 of 21 cases (48%). A recurrent pattern of aberrations was seen in cases where genetic changes were detected, involving losses at chromosome arms 1p and 9q and chromosomes 17, 19, and 22, and gains at chromosome 4 and chromosome arms 5q, 6q, 9q, and 13q. The loss of chromosome 22 was unique to younger patients (P = .05) and was associated with a higher rate of regional lymphatic metastasis (19 % vs 80 %, P = .02). Conclusions. Two genetically unique groups of patients were identified by using CGH analysis. One group had no detectable aberrations; the other had a recurrent pattern of aberrations, localizing to the identical chromosomal loci. This pattern of aberrations suggests that the involved loci may contain genes important in thyroid carcinogenesis. The clinical significance of the presence of copy number changes detected by CGH needs to be determined. In addition, molecular cloning of involved genes in each of the aberrations is warranted.
Keywords: adolescent; adult; clinical article; aged; middle aged; lymph node metastasis; chromosome 19; carcinoma, papillary; gene locus; cytogenetics; carcinogenesis; molecular cloning; chromosome aberration; thyroid neoplasms; chromosome aberrations; chromosome loss; comparative genomic hybridization; thyroid papillary carcinoma; chromosome 17; cytogenetic analysis; chromosome 6q; chromosome 1p; chromosome 22; chromosome 9q; chromosome 13q; chromosome 4; chromosome 5q; humans; human; male; female; priority journal; article
Journal Title: Surgery
Volume: 128
Issue: 6
ISSN: 0039-6060
Publisher: Elsevier Inc.  
Date Published: 2000-12-01
Start Page: 888
End Page: 894
Language: English
PUBMED: 11114620
PROVIDER: scopus
DOI: 10.1067/msy.2000.110847
Notes: Export Date: 18 November 2015 -- Source: Scopus
Citation Impact
MSK Authors
  1. Pulivarth H Rao
    66 Rao
  2. Ronald A Ghossein
    402 Ghossein
  3. Ashok R Shaha
    633 Shaha
  4. Bhuvanesh Singh
    229 Singh
  5. Robert M Tuttle
    439 Tuttle
  6. Jatin P Shah
    670 Shah
  7. Gkeok Stzuan Diana Lim
    6 Lim