Alterations of prostate biomarker expression and testosterone utilization in human LNCaP prostatic carcinoma cells by garlic-derived S-allylmercaptocysteine Journal Article

Authors: Pinto, J. T.; Qiao, C.; Xing, J.; Suffoletto, B. P.; Schubert, K. B.; Rivlin, R. S.; Huryk, R. F.; Bacich, D. J.; Heston, W. D. W.
Article Title: Alterations of prostate biomarker expression and testosterone utilization in human LNCaP prostatic carcinoma cells by garlic-derived S-allylmercaptocysteine
Abstract: BACKGROUND. This study determined the effects of S-allylmercaptocysteine (SAMC), a phytoconstituent from garlic, on the expression of androgen-responsive biomarkers, prostate specific antigen (PSA), and prostate specific membrane antigen (PSMA), in human prostatic carcinoma cells (LNCaP). METHODS. Secretion of PSA was determined as well as the activity of PSMA measured as a function of its ability to hydrolyze poly-γ-glutamated folate and N-acetylaspartylglutamate (NAAG). Folate hydrolase capacity was also determined in SAMC-treated cells grown in charcoal stripped fetal calf serum (CS-FCS). In addition, testosterone disappearance was measured from culture media of SAMC-treated LNCaP and PC-3 cells as well as from cell free lysates. RESULTS. PSA secretions were significantly decreased compared to control values at 1 day (8.4 ± 2.6 vs. 18.9 ± 1.7, P < 0.01), 4 days (18.9 ± 5.3 vs. 73.8 ± 4.4, P < 0.001), and 6 days (35.6 ± 2.1 vs. 96.5 ± 17.9 ng/105 cells, P < 0.01; mean ± SD). By contrast, PSMA activity measured as either folate hydrolase or NAAG dipeptidase (NAALADase) activity increased in cells treated with SAMC. PSMA-folate hydrolase activity in SAMC-treated cells grown in CS-FCS increased beyond that observed in cells grown in CS-FCS alone. Pre-exposure of LNCaP cells to SAMC resulted in enhanced rate of testosterone disappearance from culture media at 6 hr (P < 0.01) and at 48 hr (P < 0.001) compared to media from cells not previously exposed to SAMC. Results similar to these were also observed in androgen-independent PC-3 cells treated with SAMC. In lysates of SAMC-treated LNCaP cells, the rate of testosterone catabolism was twice that from phosphate buffered saline (PBS)-treated cells. SAMC-treated LNCaP cells grown in media supplemented with testosterone temporarily exhibited enhanced growth over a 2 day period but cell numbers declined later to levels similar to those of SAMC treatment. CONCLUSIONS. These results show that SAMC exhibits differential effects on recognized biomarkers for LNCaP cells similar to those produced by androgen deprivation and strongly suggests that this effect may be mediated, in part, by diminishing the trophic effects of testosterone, likely by converting it to metabolites less reactive toward androgen receptors. (C) 2000 Wiley-Liss, Inc.
Keywords: signal transduction; human cell; adenocarcinoma; antigen expression; cell proliferation; prostate specific antigen; cell division; tumor markers, biological; antineoplastic activity; tumor cells, cultured; enzyme activity; prostate-specific antigen; prostatic neoplasms; membrane antigen; antigens, neoplasm; cell culture; psa; epithelium cell; receptors, androgen; testosterone; drug interactions; cysteine; culture media; glutamate carboxypeptidase ii; antigens, surface; cell lysate; prostate carcinoma; plants, medicinal; growth inhibitors; receptor down regulation; radioimmunoassay; garlic; gamma-glutamyl hydrolase; psma; n acetylaspartylglutamic acid; gamma glutamyl hydrolase; biochemical marker; folate hydrolase; humans; human; male; priority journal; article; carboxypeptidases; allylsulfides; n-acetyl-α-aspartylglutamate; allyl sulfide; secretory rate
Journal Title: Prostate
Volume: 45
Issue: 4
ISSN: 0270-4137
Publisher: John Wiley & Sons  
Date Published: 2000-12-01
Start Page: 304
End Page: 314
Language: English
DOI: 10.1002/1097-0045(20001201)45:4<304::aid-pros4>;2-9
PUBMED: 11102955
PROVIDER: scopus
Notes: Export Date: 18 November 2015 -- Source: Scopus
Altmetric Score
MSK Authors
  1. Warren Heston
    97 Heston
  2. Robert   Huryk
    19 Huryk
  3. John   Pinto
    26 Pinto
  4. Richard S Rivlin
    25 Rivlin
  5. Changhong   Qiao
    7 Qiao
  6. Jie   Xing
    3 Xing
  7. Dean J Bacich
    10 Bacich