Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking Spo11 Journal Article

  


Authors: Baudat, F.; Manova, K.; Yuen, J. P.; Jasin, M.; Keeney, S.
Article Title: Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking Spo11
Abstract: Spo11, a protein first identified in yeast, is thought to generate the chromosome breaks that initiate meiotic recombination. We now report that disruption of mouse Spo11 leads to severe gonadal abnormalities from defective meiosis. Spermatocytes suffer apoptotic death during early prophase; oocytes reach the diplotene/dictyate stage in nearly normal numbers, but most die soon after birth. Consistent with a conserved function in initiating meiotic recombination, Dmc1/Rad51 focus formation is abolished. Spo11(-/-) meiocytes also display homologous chromosome synapsis defects, similar to fungi but distinct from flies and nematodes. We propose that recombination initiation precedes and is required for normal synapsis in mammals. Our results also support the view that mammalian checkpoint responses to meiotic recombination and/or synapsis defects are sexually dimorphic.
Keywords: gene deletion; dna-binding proteins; nonhuman; proteins; mouse; oocyte; spermatocyte; meiosis; animals; cell cycle proteins; mice; mice, knockout; spermatocytes; apoptosis; animal experiment; animal model; gonad dysfunction; genetic recombination; recombination, genetic; chromosome breakage; sequence homology, nucleic acid; chromosome pairing; adenosine triphosphatases; chromosomes; dna topoisomerase; esterases; gonads; oocyte development; sex characteristics; rad51 recombinase; spermatogenesis; chromosome disorder; synaptogenesis; ovarian failure, premature; male; female; article; sex differentiation; sex differentiation disorder
Journal Title: Molecular Cell
Volume: 6
Issue: 5
ISSN: 1097-2765
Publisher: Cell Press  
Date Published: 2000-11-01
Start Page: 989
End Page: 998
Language: English
PUBMED: 11106739
PROVIDER: scopus
DOI: 10.1016/S1097-2765(00)00098-8
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-0033638183&partnerID=40&md5=5242e29f2ae8ce40c5f5a366e9f680bb
Notes: Export Date: 18 November 2015 -- Source: Scopus
Altmetric
What is Altmetric?
Citation Impact
What is Dimensions Citation Badge?
BMJ Impact Analytics
MSK Authors
  1. Scott N Keeney
    138 Keeney
  2. Frederic Baudat
    12 Baudat
  3. Maria Jasin
    250 Jasin
  4. Katia O Manova-Todorova
    161 Manova-Todorova
  5. Puikuen Yuen
    1 Yuen
Related MSK Work