Recombinational DNA double-strand breaks in mice precede synapsis Journal Article
| Authors: | Mahadevaiah, S. K.; Turner, J. M. A.; Baudat, F.; Rogakou, E. P.; De Boer, P.; Blanco-Rodríguez, J.; Jasin, M.; Keeney, S.; Bonner, W. M.; Burgoyne, P. S. |
| Article Title: | Recombinational DNA double-strand breaks in mice precede synapsis |
| Abstract: | In Saccharomyces cerevisiae, meiotic recombination is initiated by Spo11-dependent double-strand breaks (DSBs), a process that precedes homologous synapsis. Here we use an antibody specific for a phosphorylated histone (γ-H2AX, which marks the sites of DSBs) to investigate the timing, distribution and Spo11-dependence of meiotic DSBs in the mouse. We show that, as in yeast, recombination in the mouse is initiated by Spo11-dependent DSBs that form during leptotene. Loss of γ-H2AX staining (which in irradiated somatic cells is temporally linked with DSB repair) is temporally and spatially correlated with synapsis, even when this synapsis is 'non-homologous'. |
| Keywords: | controlled study; dna-binding proteins; nonhuman; genetic analysis; proteins; animal cell; mouse; meiosis; animals; cell cycle proteins; mice; mice, knockout; animal tissue; animal experiment; animal model; phosphorylation; animalia; dna strand breakage; dna; saccharomyces cerevisiae; histone; recombination, genetic; microscopy, fluorescence; antibodies; chromosome pairing; histones; esterases; integrases; somatic cell genetics; recombinases; dna nucleotidyltransferases; recombinant dna; male; female; priority journal; article |
| Journal Title: | Nature Genetics |
| Volume: | 27 |
| Issue: | 3 |
| ISSN: | 1061-4036 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2001-03-01 |
| Start Page: | 271 |
| End Page: | 276 |
| Language: | English |
| DOI: | 10.1038/85830 |
| PUBMED: | 11242108 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
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