Antineuronal antibodies in patients with neuroblastoma and paraneoplastic opsoclonus-myoclonus Journal Article
| Authors: | Antunes, N. L.; Khakoo, Y.; Matthay, K. K.; Seeger, R. C.; Stram, D. O.; Gerstner, E.; Abrey, L. E.; Dalmau, J. |
| Article Title: | Antineuronal antibodies in patients with neuroblastoma and paraneoplastic opsoclonus-myoclonus |
| Abstract: | Purpose: To identify serologic markers in children with paraneoplastic opsoclonus-myoclonus (POM). Materials and Methods: We examined the sera of 64 children with neuroblastoma (16 with POM and 48 age-matched and stage-matched controls) by immunohistochemistry of rat brain and human cerebellum, and by Western blot analysis of protein extracts from human Purkinje cells, cortical neurons, neuroblastoma cell lines, and HuD. Results: Using immunohistochemistry, IgG reactivity against neurons was identified in 13 of 16 POM sera (81%), and 12 of 48 non-POM sera (25%; P < 0.001). IgM antineural antibodies were present in 3 of 16 POM sera (19%) and 11 of 48 (23%) non-POM sera. Except for anti-Hu antibodies detected in 10 sera (4 with POM), no other specific reactivities were identified by Western blot analysis of neuronal or of neuroblastoma protein extracts. Conclusions: We conclude that: 1) patients with neuroblastoma and POM are more likely to harbor antineuronal antibodies than patients without POM; 2) no specific serologic marker of POM was identified, but the frequent presence of antineuronal antibodies suggests that POM is immune-mediated; and 3) anti-Hu antibodies are present in some sera from patients with neuroblastoma, irrespective of the presence of POM. |
| Keywords: | immunohistochemistry; adult; child; controlled study; human tissue; child, preschool; major clinical study; nonhuman; animals; animal tissue; cerebellum; purkinje cells; neurons; blotting, western; immunoglobulin g; infant; infant, newborn; neuroblastoma; rat; western blotting; autoimmunity; rats; myoclonus; cerebral cortex; autoantibody; opsoclonus; paraneoplastic syndrome; autoantibodies; serology; antibodies, neoplasm; immunoglobulin m; paraneoplastic syndromes, nervous system; humans; human; male; female; priority journal; article; antineuronal antibodies |
| Journal Title: | Journal of Pediatric Hematology/Oncology |
| Volume: | 22 |
| Issue: | 4 |
| ISSN: | 1077-4114 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 2000-07-01 |
| Start Page: | 315 |
| End Page: | 320 |
| Language: | English |
| DOI: | 10.1097/00043426-200007000-00007 |
| PUBMED: | 10959901 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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