Genetic modulation of T cell receptor gene segment usage during somatic recombination Journal Article
| Authors: | Livak, F.; Burtrum, D. B.; Rowen, L.; Schatz, D. G.; Petrie, H. T. |
| Article Title: | Genetic modulation of T cell receptor gene segment usage during somatic recombination |
| Abstract: | Lymphocyte antigen receptors are not encoded by germline genes, but rather are produced by combinatorial joining between clusters of gene segments in somatic cells. Within a given cluster, gene segment usage during recombination is thought to be largely random, with biased representation in mature T lymphocytes resulting from protein-mediated selection of a subset of the total repertoire. Here we show that T cell receptor Dβ and Jβ gene segment usage is not random, but is patterned at the time of recombination. The hierarchy of gene segment usage is independent of gene segment proximity, but rather is influenced by the ability of the flanking recombination signal sequences (RSS) to bind the recombinase and/or to form a paired synaptic complex. Importantly, the relative frequency of gene segment usage established during recombination is very similar to that found after protein-mediated selection, suggesting that in addition to targeting recombinase activity, the RSS may have evolved to bias the naive repertoire in favor of useful gene products. |
| Keywords: | polymerase chain reaction; t-lymphocytes; animals; mice; somatic cell; mice, inbred c57bl; germ line; t lymphocyte receptor; kidney; genetic recombination; thymus; genes, t-cell receptor beta; recombination, genetic; signal peptide; base sequence; dna primers; t lymphocyte subpopulation; lymphocyte antigen receptor; synapse; somatic cell genetics; consensus sequence; recombinase; dna, ribosomal; vdj recombination; genes, t-cell receptor; priority journal; article; recombination signal sequence; repertoire selection; t cell receptor β locus |
| Journal Title: | Journal of Experimental Medicine |
| Volume: | 192 |
| Issue: | 8 |
| ISSN: | 0022-1007 |
| Publisher: | Rockefeller University Press |
| Date Published: | 2000-10-16 |
| Start Page: | 1191 |
| End Page: | 1196 |
| Language: | English |
| DOI: | 10.1084/jem.192.8.1191 |
| PUBMED: | 11034609 |
| PROVIDER: | scopus |
| PMCID: | PMC2195867 |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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