Mutational analysis of Escherichia coli topoisomerase IV. I. Selection of dominant-negative ParE alleles Journal Article
| Authors: | Mossessova, E.; Levine, C.; Peng, H.; Nurse, P.; Bahng, S.; Marians, K. J. |
| Article Title: | Mutational analysis of Escherichia coli topoisomerase IV. I. Selection of dominant-negative ParE alleles |
| Abstract: | In order to define regions of ParE, one of the two subunits of topoisomerase IV, that are involved in catalysis during topoisomerization, we developed a selection procedure to isolate dominant-negative parE alleles. Both wild-type parC and mutagenized parE were expressed from a tightly- regulated lac promoter on a moderate-copy plasmid. Mutated pare alleles were rescued from those plasmids that caused IPTG-dependent cell death. The mutant ParE proteins could be divided into two groups when reconstituted with ParC to form topoisomerase IV, those that elicited hyper-DNA cleavage and those that affected covalent complex formation. |
| Keywords: | promoter region; mutation; dna-binding proteins; nonhuman; mutant protein; phenotype; allele; gene expression; amino acid substitution; alleles; bacterial proteins; escherichia coli; plasmid; microscopy, fluorescence; enzyme subunit; dna topoisomerases, type ii; dna topoisomerase iv; electrophoresis, polyacrylamide gel; dna topoisomerase; dna, superhelical; enzyme reconstitution; prokaryota; isopropyl thiogalactoside; negibacteria; priority journal; article |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 275 |
| Issue: | 6 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2000-02-11 |
| Start Page: | 4099 |
| End Page: | 4103 |
| Language: | English |
| DOI: | 10.1074/jbc.275.6.4099 |
| PUBMED: | 10660569 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work