Topoisomerase III can serve as the cellular decatenase in Escherichia coli Journal Article
| Authors: | Nurse, P.; Levine, C.; Hassing, H.; Marians, K. J. |
| Article Title: | Topoisomerase III can serve as the cellular decatenase in Escherichia coli |
| Abstract: | topB, encoding topoisomerase III, was identified as a high copy suppressor of the temperature-sensitive parC1215 allele, encoding one of the subunits of topoisomerase IV. Overexpression of topoisomerase III at the nonpermissive temperature was shown subsequently to restore timely chromosome decatenation and suppress lethality in strains carrying either temperature-sensitive parE or parC alleles. By developing an assay in vitro for precatenane unlinking, we demonstrated directly that both topoisomerase III and topoisomerase IV were efficient at this task, whereas DNA gyrase was very inefficient at precatenane removal. These observations suggest that precatenane unlinking is sufficient to sustain decatenation of replicating daughter chromosomes in the cell. |
| Keywords: | unclassified drug; nonhuman; metabolism; allele; gene overexpression; enzymology; bacterial strain; bacteria (microorganisms); dna; escherichia coli; substrate specificity; fluorescence microscopy; microscopy, fluorescence; dna topoisomerase (atp hydrolysing); enzyme specificity; enzyme subunit; bacterial dna; dna, bacterial; enzymes; chromosomes; lethality; chromosome replication; dna topoisomerase iv; dna topoisomerase; dna topoisomerases, type i; prokaryota; temperature sensitive clone; dna topoisomerase iii; negibacteria; thermal effects; priority journal; article; cell enzyme; cellular decatenase; catenane; decatenase; topoisomerase iii |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 278 |
| Issue: | 10 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2003-03-07 |
| Start Page: | 8653 |
| End Page: | 8660 |
| Language: | English |
| DOI: | 10.1074/jbc.M211211200 |
| PUBMED: | 12509418 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 12 September 2014 -- Source: Scopus |
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