Identification of dnaX as a high-copy suppressor of the conditional lethal and partition phenotypes of the parE10 allele Journal Article
| Authors: | Levine, C.; Marians, K. J. |
| Article Title: | Identification of dnaX as a high-copy suppressor of the conditional lethal and partition phenotypes of the parE10 allele |
| Abstract: | Termination of DNA replication, complete topological unlinking of the parental template DNA strands, partition of the daughter chromosomes, and cell division follow in an ordered and interdependent sequence during normal bacterial growth. In Escherichia coli, topoisomerase IV (Topo IV), encoded by pare and parC, is responsible for decatenation of the two newly formed chromosomes. In an effort to uncover the pathway of information flow between the macromolecular processes that describe these events, we identified dnaX, encoding the τ and γ subunits of the DNA polymerase III holoenzyme, as a high-copy suppressor of the temperature-sensitive phenotype of the parE10 allele. We show that suppression derives from overexpression of the γ, but not the τ, subunit of the holoenzyme and that the partition defect of parE10 cells is nearly completely reverted at the nonpermissive temperature as well. These observations suggest a possible association between Topo IV and the replication machinery. |
| Keywords: | unclassified drug; nonhuman; dna replication; phenotype; gene expression; alleles; bacteria (microorganisms); bacterial proteins; escherichia coli; gene repression; temperature; dna topoisomerases, type ii; dna topoisomerase iv; dna topoisomerase; prokaryota; dna directed dna polymerase gamma; temperature sensitive mutant; negibacteria; suppression, genetic; dna polymerase iii; priority journal; article; protein dnax |
| Journal Title: | Journal of Bacteriology |
| Volume: | 180 |
| Issue: | 5 |
| ISSN: | 0021-9193 |
| Publisher: | American Society for Microbiology |
| Date Published: | 1998-03-01 |
| Start Page: | 1232 |
| End Page: | 1240 |
| Language: | English |
| PUBMED: | 9495763 |
| PROVIDER: | scopus |
| PMCID: | PMC107012 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |
