CT10: A new cancer-testis (CT) antigen homologous to CT7 and the mage family, identified by representational-difference analysis Journal Article
| Authors: | Güre, A. O.; Stockert, E.; Arden, K. C.; Boyer, A. D.; Viars, C. S.; Scanlan, M. J.; Old, L. J.; Chen, Y. T. |
| Article Title: | CT10: A new cancer-testis (CT) antigen homologous to CT7 and the mage family, identified by representational-difference analysis |
| Abstract: | Assays relying on humoral or T-cell-based recognition of tumor antigens to identify potential targets for immunotherapy have led to the discovery of a significant number of immunogenic gene products, including cancer-testis (CT) antigens predominantly expressed in cancer cells and male germ cells. The search for cancer-specific antigens has been extended via the technique of representational-difference analysis and SK-MEL-37, a melanoma cell line expressing a broad range of CT antigens. Using this approach, we have isolated CT antigen genes, genes over-expressed in cancer, e.g., PRAME and KOC, and genes encoding neuro-ectodermal markers. The identified CT antigen genes include the previously defined MAGE-A6, MAGE-A4a, MAGE-A10, CT7/MAGE- CI, as well as a novel gene designated CT10, which shows strong homology to CT7/MAGE-CI both at cDNA and at genomic levels. Chromosome mapping localized CT10 to Xq27, in close proximity to CT7/MAGE-CI and MAGE-A genes. CT10 mRNA is expressed in testis and in 20 to 30% of various human cancers. A serological survey identified 2 melanoma patients with anti-CT10 antibody, demonstrating the immunogenicity of CT10 in humans. (C) 2000 Wiley-Liss, Inc. |
| Keywords: | protein expression; human cell; exons; protein localization; gene overexpression; melanoma; neoplasm proteins; introns; transcription, genetic; tumor cells, cultured; tumor antigen; carcinogenesis; gene expression regulation, neoplastic; amino acid sequence; molecular sequence data; sequence homology, amino acid; antigens, neoplasm; messenger rna; rna, messenger; sequence alignment; organ specificity; base sequence; genome; testis; complementary dna; chromosome map; chromosome mapping; gene isolation; cancer; humans; human; male; female; priority journal; article |
| Journal Title: | International Journal of Cancer |
| Volume: | 85 |
| Issue: | 5 |
| ISSN: | 0020-7136 |
| Publisher: | John Wiley & Sons |
| Date Published: | 2000-03-01 |
| Start Page: | 726 |
| End Page: | 732 |
| Language: | English |
| DOI: | 10.1002/(sici)1097-0215(20000301)85:5<726::aid-ijc21>3.0.co;2-f |
| PUBMED: | 10699956 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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