Engraftment and growth of patient-derived retinoblastoma tumour in severe combined immunodeficiency mice Journal Article


Authors: Yan, Y.; Dunkel, I. J.; Guan, X.; Abramson, D. H.; Jhanwar, S. C.; O'Reilly, R. J.
Article Title: Engraftment and growth of patient-derived retinoblastoma tumour in severe combined immunodeficiency mice
Abstract: The development of an in vivo model of retinoblastoma could be important for studying its biological behaviour and developing novel therapeutic strategies. We examined the ability of patient-derived retinoblastoma cells to grow and disseminate in severe combined immunodeficiency CB-17-SCID mice after subcutaneous (s.c.) inoculation without conditioning treatment. 24/30 (80%) of patient-derived tumours engrafted and grew as s.c. nodules in SCID mice. Whilst most xenografted tumours appeared to be localised, by PCR assay a positive DNA band of human minisatellite region (YNZ.22) was determined in the bone marrow of 19/25 (76%), in the spleen of 14/25 (56%) and in the liver of 16/25 (64%) mice, respectively, indicating dissemination to distant organs. Cytogenetic analysis demonstrated i(6p) in 5/12 (42%) and trisomy 1 or 1q abnormalities in 8/12 (67%) of the xenografted tumour samples studied, respectively, suggesting that retinoblastoma tumour cells maintain their cytogenetic abnormalities following adoptive growth in SCID mice. In this report we demonstrate the ability to propagate human primary retinoblastoma cells in SCID mice after s.c. inoculation and suggest the possibility of using the SCID mouse model to study the intrinsic biological behaviour of human retinoblastoma and to develop novel therapeutic strategies in the treatment of this disease. (C) 2000 Elsevier Science Ltd.
Keywords: child; child, preschool; nonhuman; polymerase chain reaction; chromosome 1; mouse; animals; mice; animal tissue; metastasis; animal experiment; animal model; retinoblastoma; mice, scid; retinal neoplasms; infant; chromosomes, human, pair 6; neoplasm transplantation; chromosome aberrations; chromosomes, human, pair 1; karyotyping; combined immunodeficiency; chromosome 1q; chromosomes, human, pair 16; trisomy; chromosome inversion; cancer graft; humans; male; female; priority journal; article; scid mice; human retinoblastoma; in vivo growth pattern; dna minisatellite
Journal Title: European Journal of Cancer
Volume: 36
Issue: 2
ISSN: 0959-8049
Publisher: Elsevier Inc.  
Date Published: 2000-01-01
Start Page: 221
End Page: 228
Language: English
DOI: 10.1016/s0959-8049(99)00277-4
PUBMED: 10741281
PROVIDER: scopus
DOI/URL:
Notes: Export Date: 18 November 2015 -- Source: Scopus
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  1. Ira J Dunkel
    371 Dunkel
  2. Suresh C Jhanwar
    293 Jhanwar
  3. Richard O'Reilly
    748 O'Reilly
  4. Xiuqin   Guan
    5 Guan
  5. Ying   Yan
    13 Yan