Administration of rabbit anti-asialo GM1 antiserum facilitates the development of human Epstein-Barr virus-induced lymphoproliferations in xenografted C.B-17 scid/scid mice Journal Article

  

Authors:	Lacerda, J. F.; Ladanyi, M.; Jagiello, C.; O'Reilly, R. J.
Article Title:	Administration of rabbit anti-asialo GM1 antiserum facilitates the development of human Epstein-Barr virus-induced lymphoproliferations in xenografted C.B-17 scid/scid mice
Abstract:	Mice with severe combined immune deficiency (C.B-17 scid/scid [SCID mice]) lack functional B and T lymphocytes and are permissive for the growth of human xenografts. However, the development of functional NK cells is not affected by the scid mutation. Mouse NK cells express the surface glycolipid asialo GM1 and are implicated in the rejection of heterotransplanted cells. In the present study, we demonstrate that SCID mice treated with rabbit anti- asialo GM1 antiserum (α-asialo GM1), for in vivo depletion of endogenous NK cell function, develop lethal Epstein-Barr virus (EBV)-induced lymphoproliferative disorders (EBV-LPD) at lower doses of inoculated EBV- transformed lymphoblastoid B cell lines (EBV-LCL) than untreated animals. Furthermore, at any given dose of EBV-LCL inoculated, EBV-LPD developed earlier and induced lethality sooner in α-asialo GM1-treated animals. We also demonstrate that SCID mice treated with α-asialo GM1 have a reduction in the number of asialo GM1-expressing splenocytes. Moreover, splenic cell suspensions derived from α-asialo GM1-treated SCID mice show lower cytotoxicity against the mouse NK-sensitive cell line YAC-1 and human EBV- LCL than splenocytes obtained from untreated SCID mice.
Keywords:	nonhuman; t lymphocyte; animal cell; mouse; animals; mice; animal tissue; spleen; animal model; mice, scid; b lymphocyte; b-lymphocytes; xenograft; transplantation, heterologous; natural killer cell; killer cells, natural; cytotoxicity, immunologic; lymphoproliferative disease; antibodies; antibody; combined immunodeficiency; epstein barr virus; cell transformation, viral; herpesvirus 4, human; lymphoproliferative disorders; rabbits; g(m1) ganglioside; humans; priority journal; article; gangliotetraosylceramide
Journal Title:	Transplantation
Volume:	61
Issue:	3
ISSN:	0041-1337
Publisher:	Lippincott Williams & Wilkins
Date Published:	1996-02-15
Start Page:	492
End Page:	497
Language:	English
DOI:	10.1097/00007890-199602150-00030
PUBMED:	8610366
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030065334&doi=10.1097%2f00007890-199602150-00030&partnerID=40&md5=69de3a22004cd4a1ab845a7ccb6fa458
Notes:	Article -- Export Date: 22 November 2017 -- Source: Scopus

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