An apparent interlocus gene conversion-like event at a putative tumor suppressor gene locus on human chromosome 6q27 in a Burkitt's lymphoma cell line Journal Article
| Authors: | Hauptschein, R. S.; Gaidano, G.; Rao, P. H.; Scotto, L.; Edwards, Y. H.; Chaganti, R. S. K.; Dalla-Favera, R. |
| Article Title: | An apparent interlocus gene conversion-like event at a putative tumor suppressor gene locus on human chromosome 6q27 in a Burkitt's lymphoma cell line |
| Abstract: | A region of minimal deletion in B-cell non-Hodgkin's lymphoma (B-NHL) has recently been defined between D6S186 and D6S227 spanning 5-9 Mb at 6q26-q27, predicting the presence of at least one tumor suppressor gene (TSG) at this locus. During the construction of a deletion map in the B-NHL tumor panel, we report the identification of a Burkitt's lymphoma cell line, BL74, having an apparent homozygous deletion at the D6S347 locus, internal to the critical region. Since this case may facilitate the localization of the target TSG, a detailed structural molecular characterization and search for candidate genes were undertaken at this locus. While BL74 underwent a loss of heterozygosity at 6q26-q27, D6S347 was also likely subjected to a somatic interlocus gene conversion-like event between two homologous but distinct loci, resulting in the homozygous replacement of a 1860-to 2067-bp segment of one locus with the corresponding segment copied from the other locus. Two genes at this locus were identified, but their lack of expression in B-cell lineages tentatively excludes them as candidate TSGs. Another still unidentified gene at this locus may be disrupted by the gene conversion-like event, which would represent a novel mechanism of TSG inactivation. |
| Keywords: | exon; gene deletion; genetics; exons; molecular genetics; metabolism; allele; biological model; alleles; genetic transcription; transcription, genetic; tumor cells, cultured; rna; gene conversion; tumor suppressor gene; molecular sequence data; cell culture; nucleotide sequence; base sequence; chromosomes, human, pair 6; models, genetic; dna sequence; heterozygosity loss; sequence homology; sequence homology, nucleic acid; loss of heterozygosity; blotting, northern; chromosome 6; burkitt lymphoma; genes, tumor suppressor; chromosome map; pulsed field gel electrophoresis; chromosome mapping; electrophoresis, gel, pulsed-field; northern blotting; sequence analysis, dna; southern blotting; restriction fragment length polymorphism; polymorphism, restriction fragment length; blotting, southern; humans; human; article; burkitt's lymphoma; chromosome 6q27; tumor suppressor locus |
| Journal Title: | DNA Research |
| Volume: | 7 |
| Issue: | 4 |
| ISSN: | 1340-2838 |
| Publisher: | Oxford University Press |
| Date Published: | 2000-01-01 |
| Start Page: | 261 |
| End Page: | 272 |
| Language: | English |
| PUBMED: | 10997876 |
| PROVIDER: | scopus |
| DOI: | 10.1093/dnares/7.4.261 |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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