Structure of a c-Cbl-UbcH7 complex: RING domain function in ubiquitin-protein ligases Journal Article
| Authors: | Zheng, N.; Wang, P.; Jeffrey, P. D.; Pavletich, N. P. |
| Article Title: | Structure of a c-Cbl-UbcH7 complex: RING domain function in ubiquitin-protein ligases |
| Abstract: | Ubiquitin-protein ligases (E3s) regulate diverse cellular processes by mediating protein ubiquitination. The c-Cbl proto-oncogene is a RING family E3 that recognizes activated receptor tyrosine kinases, promotes their ubiquitination by a ubiquitin-conjugating enzyme (E2) and terminates signaling. The crystal structure of c-Cbl bound to a cognate E2 and a kinase peptide shows how the RING domain recruits the E2. A comparison with a HECT family E3-E2 complex indicates that a common E2 motif is recognized by the two E3 families. The structure reveals a rigid coupling between the peptide binding and the E2 binding domains and a conserved surface channel leading from the peptide to the E2 active site, suggesting that RING E3s may function as scaffolds that position the substrate and the E2 optimally for ubiquitin transfer. |
| Keywords: | signal transduction; proto-oncogene proteins; nonhuman; ubiquitin; protein conformation; protein domain; animals; proto oncogene; ubiquitin protein ligase; protein degradation; protein binding; drosophila; protein tyrosine kinase; structure-activity relationship; amino acid sequence; molecular sequence data; molecular recognition; models, molecular; caenorhabditis elegans; protein structure; proto-oncogene proteins c-cbl; enzyme substrate complex; ubiquitin-conjugating enzymes; ubiquitin-protein ligases; molecular conformation; protein modification; macromolecular substances; enzyme active site; ubiquitin conjugating enzyme; ligases; humans; priority journal; article |
| Journal Title: | Cell |
| Volume: | 102 |
| Issue: | 4 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 2000-08-18 |
| Start Page: | 533 |
| End Page: | 539 |
| Language: | English |
| PUBMED: | 10966114 |
| PROVIDER: | scopus |
| DOI: | 10.1016/S0092-8674(00)00057-X |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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