Chromosome missegregation and apoptosis in mice lacking the mitotic checkpoint protein Mad2 Journal Article

  


Authors: Dobles, M.; Liberal, V.; Scott, M. L.; Benezra, R.; Sorger, P. K.
Article Title: Chromosome missegregation and apoptosis in mice lacking the mitotic checkpoint protein Mad2
Abstract: The initiation of chromosome segregation at anaphase is linked by the spindle assembly checkpoint to the completion of chromosome-microtubule attachment during metaphase. To determine the function of the mitotic checkpoint protein Mad2 during normal cell division and when mitosis goes awry, we have knocked out Mad2 in mice. We find that E5.5 embryonic cells lacking Mad2, like mad2 yeast, grow normally but are unable to arrest in response to spindle disruption. At E6.5, the cells of the epiblast begin rapid cell division and the absence of a checkpoint results in widespread chromosome missegregation and apoptosis. In contrast, the postmitotic trophoblast giant cells survive without Mad2. Thus, the spindle assembly checkpoint is required for accurate chromosome segregation in mitotic mouse cells, and for embryonic viability, even in the absence of spindle damage.
Keywords: nonhuman; mitosis; animal cell; chromosome; mouse; animals; cell cycle proteins; mice; mice, knockout; gene; reverse transcription polymerase chain reaction; apoptosis; embryo; protein; animalia; nuclear proteins; rna; gene expression regulation; dna; amino acid sequence; molecular sequence data; sequence homology, amino acid; carrier proteins; dna sequence; calcium-binding proteins; metaphase; chromosome segregation; fungal proteins; priority journal; article; goes
Journal Title: Cell
Volume: 101
Issue: 6
ISSN: 0092-8674
Publisher: Cell Press  
Date Published: 2000-06-09
Start Page: 635
End Page: 645
Language: English
PUBMED: 10892650
PROVIDER: scopus
DOI: 10.1016/S0092-8674(00)80875-2
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-0034625268&partnerID=40&md5=29cc45396df3dbecd95e5c3b1da62e62
Notes: Export Date: 18 November 2015 -- Source: Scopus
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MSK Authors
  1. Robert Benezra
    146 Benezra
  2. Vasco Liberal
    4 Liberal
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