Identification of a human mitotic checkpoint gene: hsMAD2 Journal Article
| Authors: | Li, Y.; Benezra, R. |
| Article Title: | Identification of a human mitotic checkpoint gene: hsMAD2 |
| Abstract: | In Saccharomyces cerevisiae, MAD2 is required for mitotic arrest if the spindle assembly is perturbed. The human homolog of MAD2 was isolated and shown to be a necessary component of the mitotic checkpoint in HeLa cells by antibody electroporation experiments. Human, or Homo sapiens, MAD2 (hsMAD2) was localized at the kinetochore after chromosome condensation but was no longer observed at the kinetochore in metaphase, suggesting that MAD2 might monitor the completeness of the spindle-kinetochore attachment. Finally, T47D, a human breast tumor cell line that is sensitive to taxol and nocodazole, had reduced MAD2 expression and failed to arrest in mitosis after nocodazole treatment. Thus, defects in the mitotic checkpoint may contribute to the sensitivity of certain tumors to mitotic spindle inhibitors. |
| Keywords: | human cell; paclitaxel; mitosis; cell cycle proteins; gene expression; protein; tumor cells, cultured; hela cell; hela cells; amino acid sequence; molecular sequence data; saccharomyces cerevisiae; carrier proteins; tumor cell line; electroporation; anaphase; mitosis spindle; mitotic spindle apparatus; calcium-binding proteins; repressor proteins; drug sensitivity; metaphase; gene location; complementary dna; centromere; nocodazole; interphase; kinetochores; mitosis inhibition; gene isolation; chromosome condensation; homo sapiens; humans; human; priority journal; article |
| Journal Title: | Science |
| Volume: | 274 |
| Issue: | 5285 |
| ISSN: | 0036-8075 |
| Publisher: | American Association for the Advancement of Science |
| Date Published: | 1996-10-11 |
| Start Page: | 246 |
| End Page: | 248 |
| Language: | English |
| DOI: | 10.1126/science.274.5285.246 |
| PUBMED: | 8824189 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
