Levels of E2F-1 expression are higher in lung metastasis of colon cancer as compared with hepatic metastasis and correlate with levels of thymidylate synthase Journal Article
| Authors: | Banerjee, D.; Gorlick, R.; Liefshitz, A.; Danenberg, K.; Danenberg, P. C.; Danenberg, P. V.; Klimstra, D.; Jhanwar, S.; Cordon-Cardo, C.; Fong, Y.; Kemeny, N.; Bertino, J. R. |
| Article Title: | Levels of E2F-1 expression are higher in lung metastasis of colon cancer as compared with hepatic metastasis and correlate with levels of thymidylate synthase |
| Abstract: | We recently reported that forced overexpression of the transcription factor E2F-1 in human HT-1080 fibrosarcoma cells resulted in corresponding high levels of thymidylate synthase (TS) and resistance to 5- fluoropyrimidines (D. Banerjee et al., Cancer Res., 58: 4292-4296, 1998). Because colorectal metastasis to the lung has higher TS levels than liver metastasis and is less responsive to treatment with 5-fluorouracil (R. Gorlick et al., J. Clin. Oncol., 16. 1465-1469, 1998), it was, therefore, of interest to measure E2F-1 expression in these tumors. In contrast to marginally increased levels of dihydrofolate reductase and topoisomerase I in lung metastasis as compared with liver metastasis, lung tumors had a 5-fold increase in E2F-1 expression as compared with liver tumors, corresponding to the relative levels of TS in these metastases. These data indicate that there exists a close correlation between E2F-1 and TS levels and provide a rationale for targeting this transcription factor, i.e., E2F-1, for the treatment of certain cancers. |
| Keywords: | immunohistochemistry; controlled study; human cell; dna-binding proteins; fluorouracil; liver neoplasms; adenocarcinoma; cell cycle proteins; gene overexpression; reverse transcription polymerase chain reaction; protein targeting; lung neoplasms; colonic neoplasms; transcription factors; cancer resistance; liver metastasis; lung metastasis; blotting, western; fibrosarcoma; reverse transcriptase polymerase chain reaction; colon cancer; transcription factor e2f; carrier proteins; western blotting; dihydrofolate reductase; thymidylate synthase; enzyme release; fluoropyrimidine; dna topoisomerase; transcription factor dp1; e2f1 transcription factor; e2f transcription factors; humans; human; priority journal; article |
| Journal Title: | Cancer Research |
| Volume: | 60 |
| Issue: | 9 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 2000-05-01 |
| Start Page: | 2365 |
| End Page: | 2367 |
| Language: | English |
| PUBMED: | 10811110 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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