Radiation-induced apoptosis of endothelial cells in the murine central nervous system: Protection by fibroblast growth factor and sphingomyelinase deficiency Journal Article
| Authors: | Peña, L. A.; Fuks, Z.; Kolesnick, R. N. |
| Article Title: | Radiation-induced apoptosis of endothelial cells in the murine central nervous system: Protection by fibroblast growth factor and sphingomyelinase deficiency |
| Abstract: | Injury to the central nervous system (CNS) by ionizing radiation may be a consequence of damage to the vascular endothelium. Recent studies showed that radiation-induced apoptosis of endothelial cells in vitro and in the lung in vivo is mediated by the lipid second messenger ceramide via activation of acid sphingomyelinase (ASM). This apoptotic response to radiation can be inhibited by basic fibroblast growth factor or by genetic mutation of ASM. In the CNS, single-dose radiation has been shown to result in a 15% loss of endothelial cells within 24 h, but whether or not this loss is associated with apoptosis remains unknown. In the present studies, dose- and time-dependent induction of apoptosis was observed in the C57BL/6 mouse CNS. Apoptosis was quantified by terminal deoxynucleotidyl transferase- mediated nick end labeling, and specific endothelial apoptosis was determined by histochemical double labeling with terminal deoxynucleotidyl transferase- mediated nick end labeling and Lycopersicon esculentum lectin. Beginning at 4 h after single-dose radiation, apoptosis was ongoing for 24 h and peaked at 12 h at an incidence of 0.7-1.4% of the total cells in spinal cord sections. Up to 20% of the apoptotic cells were endothelial. This effect was also seen in multiple regions of the brain (medulla, pons, and hippocampus). A significant reduction of radiation-induced apoptosis was observed after i.v. basic fibroblast growth factor treatment (0.45-4.5 μg/mouse). Identical results were noted in C3H/HeJ mice. Furthermore, irradiated ASM knockout mice displayed as much as a 70% reduction in endothelial apoptosis. This study demonstrates that ionizing radiation induces early endothelial cell apoptosis throughout the CNS. These data are consistent with recent evidence linking radiation-induced stress with ceramide and suggest approaches to modify the apoptotic response in control of radiation toxicity in the CNS. |
| Keywords: | gene mutation; nonhuman; mouse; animals; mice; mice, knockout; animal tissue; apoptosis; animal experiment; animal model; dose-response relationship, drug; enzyme activity; radiation injury; mice, inbred c57bl; central nervous system; endothelium cell; mice, inbred strains; fibroblast growth factor 2; vascular endothelium; endothelium, vascular; brain; spinal cord; blood vessel injury; histochemistry; ionizing radiation; dna nucleotidylexotransferase; cerebrovascular circulation; fibroblast growth factor; fibroblast culture; whole-body irradiation; sphingomyelin phosphodiesterase; mice, inbred c3h; hippocampus; microglia; in situ nick-end labeling; niemann pick disease; cesium radioisotopes; male; priority journal; article; ceramide glucosyltransferase inhibitor |
| Journal Title: | Cancer Research |
| Volume: | 60 |
| Issue: | 2 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 2000-01-15 |
| Start Page: | 321 |
| End Page: | 327 |
| Language: | English |
| PUBMED: | 10667583 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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