POS-1 promotes endo-mesoderm development by inhibiting the cytoplasmic polyadenylation of neg-1 mRNA Journal Article

   

Authors:	Elewa, A.; Shirayama, M.; Kaymak, E.; Harrison, P. F.; Powell, D. R.; Du, Z.; Chute, C. D.; Woolf, H.; Yi, D.; Ishidate, T.; Srinivasan, J.; Bao, Z.; Beilharz, T. H.; Ryder, S. P.; Mello, C. C.
Article Title:	POS-1 promotes endo-mesoderm development by inhibiting the cytoplasmic polyadenylation of neg-1 mRNA
Abstract:	The regulation of mRNA translation is of fundamental importance in biological mechanisms ranging from embryonic axis specification to the formation of long-term memory. POS-1 is one of several CCCH zinc-finger RNA-binding proteins that regulate cell fate specification during C. elegans embryogenesis. Paradoxically, pos-1 mutants exhibit striking defects in endo-mesoderm development but have wild-type distributions of SKN-1, a key determinant of endo-mesoderm fates. RNAi screens for pos-1 suppressors identified genes encoding the cytoplasmic poly(A)-polymerase homolog GLD-2, the Bicaudal-C homolog GLD-3, and the protein NEG-1. We show that NEG-1 localizes in anterior nuclei, where it negatively regulates endo-mesoderm fates. In posterior cells, POS-1 binds the neg-1 3' UTR to oppose GLD-2 and GLD-3 activities that promote NEG-1 expression and cytoplasmic lengthening of the neg-1 mRNA poly(A) tail. Our findings uncover an intricate series of post-transcriptional regulatory interactions that, together, achieve precise spatial expression of endo-mesoderm fates in C. elegans embryos. The RNA-binding protein POS-1 promotes endo-mesoderm development in C. elegans embryos. © 2015 Elsevier Inc.
Keywords:	controlled study; protein expression; unclassified drug; nonhuman; gene expression; protein binding; cell fate; drosophila; embryo development; cell differentiation; in vitro study; fluorescence polarization; rna binding protein; in situ hybridization; gene interaction; rna translation; 3' untranslated region; caenorhabditis elegans; gene regulatory network; mesoderm; nuclear localization signal; endoderm; open reading frame; polyadenylation; priority journal; article; neg 1 protein; pos 1 protein
Journal Title:	Developmental Cell
Volume:	34
Issue:	1
ISSN:	1534-5807
Publisher:	Cell Press
Date Published:	2015-07-06
Start Page:	108
End Page:	118
Language:	English
DOI:	10.1016/j.devcel.2015.05.024
PROVIDER:	scopus
PMCID:	PMC4507413
PUBMED:	26096734
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84943276056&partnerID=40&md5=4d9d70168f189b46cd30dcf50d35a817
Notes:	Export Date: 2 November 2015 -- Source: Scopus

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