The FXR1 network acts as a signaling scaffold for actomyosin remodeling Journal Article
| Authors: | Chen, X.; Fansler, M. M.; Janjoš, U.; Ule, J.; Mayr, C. |
| Article Title: | The FXR1 network acts as a signaling scaffold for actomyosin remodeling |
| Abstract: | It is currently not known whether mRNAs fulfill structural roles in the cytoplasm. Here, we report the fragile X-related protein 1 (FXR1) network, an mRNA-protein (mRNP) network present throughout the cytoplasm, formed by FXR1-mediated packaging of exceptionally long mRNAs. These mRNAs serve as an underlying condensate scaffold and concentrate FXR1 molecules. The FXR1 network contains multiple protein binding sites and functions as a signaling scaffold for interacting proteins. We show that it is necessary for RhoA signaling-induced actomyosin reorganization to provide spatial proximity between kinases and their substrates. Point mutations in FXR1, found in its homolog FMR1, where they cause fragile X syndrome, disrupt the network. FXR1 network disruption prevents actomyosin remodeling-an essential and ubiquitous process for the regulation of cell shape, migration, and synaptic function. Our findings uncover a structural role for cytoplasmic mRNA and show how the FXR1 RNA-binding protein as part of the FXR1 network acts as an organizer of signaling reactions. |
| Keywords: | gene; phosphorylation; rna-binding proteins; specificity; messenger-rna; domains; fragile-x-syndrome; nonmuscle myosin-ii; coiled-coil; contains |
| Journal Title: | Cell |
| Volume: | 187 |
| Issue: | 18 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 2024-09-05 |
| Start Page: | 5048 |
| End Page: | 5063.e25 |
| Language: | English |
| ACCESSION: | WOS:001309035900001 |
| DOI: | 10.1016/j.cell.2024.07.015 |
| PROVIDER: | wos |
| PMCID: | PMC11380585 |
| PUBMED: | 39106863 |
| Notes: | Article -- MSK Cancer Center Support Grant (P30 CA008748) acknowledged in PubMed and PDF -- MSK corresponding author is Christine Mayr -- Source: Wos |
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