The Drosophila fragile X protein functions as a negative regulator in the orb autoregulatory pathway Journal Article
| Authors: | Costa, A.; Wang, Y.; Dockendorff, T. C.; Erdjument-Bromage, H.; Tempst, P.; Schedl, P.; Jongens, T. A. |
| Article Title: | The Drosophila fragile X protein functions as a negative regulator in the orb autoregulatory pathway |
| Abstract: | Translational regulation of maternal mRNAs in distinct temporal and spatial patterns underlies many key decisions in developing eggs and embryos. In Drosophila, Orb is responsible for mediating the translational activation of mRNAs localized within the developing oocyte. Orb is a germline-specific RNA binding protein and is one of the founding members of the CPEB family of translational regulators. Here we show that Orb associates with the Drosophila Fragile X Mental Retardation (dFMR1) protein as part of a ribonucleoprotein complex that controls the localized translation of mRNAs in developing egg chambers. One of the key orb regulatory targets is orb mRNA, and this autoregulatory activity is critical for ensuring that Orb protein is expressed at high levels in the oocyte. We show that dFMR1 functions as a negative regulator in the orb autoregulatory circuit, downregulating orb mRNA translation. Copyright © 2005 by Elsevier Inc. |
| Keywords: | controlled study; protein expression; unclassified drug; nonhuman; protein function; protein localization; animals; oocytes; protein binding; drosophila; embryo development; rna binding protein; rna-binding proteins; messenger rna; rna, messenger; gene interaction; protein biosynthesis; gene control; down regulation; drosophila melanogaster; drosophila proteins; protein determination; autoregulation; oocyte development; oogenesis; translation regulation; ribonucleoprotein; fragile x mental retardation protein; cytoplasmic polyadenylation element binding protein; protein orb |
| Journal Title: | Developmental Cell |
| Volume: | 8 |
| Issue: | 3 |
| ISSN: | 1534-5807 |
| Publisher: | Cell Press |
| Date Published: | 2005-03-08 |
| Start Page: | 331 |
| End Page: | 342 |
| Language: | English |
| DOI: | 10.1016/j.devcel.2005.01.011 |
| PUBMED: | 15737929 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 42" - "Export Date: 24 October 2012" - "CODEN: DCEEB" - "Source: Scopus" |
