Applications of targeted proteomics in systems biology and translational medicine Journal Article

Authors: Ebhardt, H. A.; Root, A.; Sander, C.; Aebersold, R.
Article Title: Applications of targeted proteomics in systems biology and translational medicine
Abstract: Biological systems are composed of numerous components of which proteins are of particularly high functional significance. Network models are useful abstractions for studying these components in context. Network representations display molecules as nodes and their interactions as edges. Because they are difficult to directly measure, functional edges are frequently inferred from suitably structured datasets consisting of the accurate and consistent quantification of network nodes under a multitude of perturbed conditions. For the precise quantification of a finite list of proteins across a wide range of samples, targeted proteomics exemplified by selected/multiple reaction monitoring (SRM, MRM) mass spectrometry has proven useful and has been applied to a variety of questions in systems biology and clinical studies. Here, we survey the literature of studies using SRM-MS in systems biology and clinical proteomics. Systems biology studies frequently examine fundamental questions in network biology, whereas clinical studies frequently focus on biomarker discovery and validation in a variety of diseases including cardiovascular disease and cancer. Targeted proteomics promises to advance our understanding of biological networks and the phenotypic significance of specific network states and to advance biomarkers into clinical use. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Keywords: signal transduction; protein expression; review; nonhuman; neoplasm; multiple reaction monitoring; mass spectrometry; biological marker; protein analysis; complex formation; protein protein interaction; protein; proteomics; keratinocyte; protein processing; saccharomyces cerevisiae; cardiovascular disease; cellular distribution; eukaryotic cell; protein structure; reaction analysis; antibody affinity; systems biology; prokaryote; clinical proteomics; human; priority journal; targeted proteomics; selected reaction monitoring
Journal Title: Proteomics
Volume: 15
Issue: 18
ISSN: 1615-9853
Publisher: Wiley V C H Verlag Gmbh  
Date Published: 2015-09-01
Start Page: 3193
End Page: 3208
Language: English
DOI: 10.1002/pmic.201500004
PROVIDER: scopus
PUBMED: 26097198
PMCID: PMC4758406
Notes: Export Date: 2 October 2015 -- Source: Scopus
Citation Impact
MSK Authors
  1. Chris Sander
    205 Sander
  2. Alex Root
    7 Root