Protein structure determination by combining sparse NMR data with evolutionary couplings Journal Article
| Authors: | Tang, Y.; Huang, Y. J.; Hopf, T. A.; Sander, C.; Marks, D. S.; Montelione, G. T. |
| Article Title: | Protein structure determination by combining sparse NMR data with evolutionary couplings |
| Abstract: | Accurate determination of protein structure by NMR spectroscopy is challenging for larger proteins, for which experimental data are often incomplete and ambiguous. Evolutionary sequence information together with advances in maximum entropy statistical methods provide a rich complementary source of structural constraints. We have developed a hybrid approach (evolutionary coupling-NMR spectroscopy; EC-NMR) combining sparse NMR data with evolutionary residue-residue couplings and demonstrate accurate structure determination for several proteins 6-41 kDa in size. © 2015 Nature America, Inc. All rights reserved. |
| Keywords: | protein domain; protein analysis; amino acid sequence; nuclear magnetic resonance spectroscopy; protein structure; protein determination; proton transport; priority journal; article |
| Journal Title: | Nature Methods |
| Volume: | 12 |
| Issue: | 8 |
| ISSN: | 1548-7091 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2015-08-01 |
| Start Page: | 751 |
| End Page: | 754 |
| Language: | English |
| DOI: | 10.1038/nmeth.3455 |
| PROVIDER: | scopus |
| PMCID: | PMC4521990 |
| PUBMED: | 26121406 |
| DOI/URL: | |
| Notes: | Export Date: 2 September 2015 -- Source: Scopus |
