Residual dipolar couplings: Synergy between NMR and structural genomics Journal Article

Authors: Al-Hashimi, H. M.; Patel, D. J.
Article Title: Residual dipolar couplings: Synergy between NMR and structural genomics
Abstract: Structural genomics is on a quest for the structure and function of a significant fraction of gene products. Current efforts are focusing on structure determination of single-domain proteins, which can readily be targeted by X-ray crystallography, NMR spectroscopy and computational homology modeling. However, comprehensive association of gene products with functions also requires systematic determination of more complex protein structures and other biomolecules participating in cellular processes such as nucleic acids, and characterization of biomolecular interactions and dynamics relevant to function. Such NMR investigations are becoming more feasible, not only due to recent advances in NMR methodology, but also because structural genomics is providing valuable structural information and new experimental and computational tools. The measurement of residual dipolar couplings in partially oriented systems and other new NMR methods will play an important role in this synergistic relationship between NMR and structural genomics. Both an expansion in the domain of NMR application, and important contributions to future structural genomics efforts can be anticipated.
Keywords: controlled study; review; methodology; protein conformation; protein domain; protein function; proteome; animal; animals; molecular dynamics; gene product; gene function; protein interaction; structure activity relation; structure-activity relationship; proteomics; chemistry; nuclear magnetic resonance spectroscopy; genomics; protein structure; sequence homology; x ray crystallography; protein determination; nuclear magnetic resonance; chemical reaction; molecular model; gene structure; dipole; magnetism; magnetics; nucleic acid; nuclear magnetic resonance, biomolecular; humans; human; priority journal; article; support, u.s. gov't, p.h.s.; dynamics and function; long-range constraints; multi-domain proteins; rna tertiary structure
Journal Title: Journal of Biomolecular NMR
Volume: 22
Issue: 1
ISSN: 0925-2738
Publisher: Springer  
Date Published: 2002-01-01
Start Page: 1
End Page: 8
Language: English
DOI: 10.1023/a:1013801714041
PUBMED: 11885976
PROVIDER: scopus
Notes: Export Date: 14 November 2014 -- Source: Scopus
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MSK Authors
  1. Dinshaw J Patel
    392 Patel